Arcobacter spp. - modern method for detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900312" target="_blank" >RIV/00216275:25310/15:39900312 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Arcobacter spp. - modern method for detection

Popis výsledku v původním jazyce

This chapter focuses on detecting biofilm and planktonic bacterial cells of the genera Arcobacter and Campylobacter. These bacteria can cause a lot of diseases. Especially, in the last decade, arcobacters have been increasingly isolated from feces of clinically healthy and ill animals, foods of animal origin and various types of water. There are several modern methods for bacteria detection which can be suitable tool. A multiplex-polymerase chain reaction (PCR) method was introduced and optimized for detecting biofilm while using the intercalating dyes ethidium monoazide (EMA) and propidium monoazide (PMA) for collection strains of bacteria of the genus Arcobacter with subsequent application on samples of prepared biofilms. Fluorescence in situ hybridization was used to detect biofilm and planktonic cells of selected microorganisms. This method was optimized and subsequently applied to biofilm samples prepared on stainless steel coupons. The study results indicate that fluorescence in

Název v anglickém jazyce

Arcobacter spp. - modern method for detection

Popis výsledku anglicky

This chapter focuses on detecting biofilm and planktonic bacterial cells of the genera Arcobacter and Campylobacter. These bacteria can cause a lot of diseases. Especially, in the last decade, arcobacters have been increasingly isolated from feces of clinically healthy and ill animals, foods of animal origin and various types of water. There are several modern methods for bacteria detection which can be suitable tool. A multiplex-polymerase chain reaction (PCR) method was introduced and optimized for detecting biofilm while using the intercalating dyes ethidium monoazide (EMA) and propidium monoazide (PMA) for collection strains of bacteria of the genus Arcobacter with subsequent application on samples of prepared biofilms. Fluorescence in situ hybridization was used to detect biofilm and planktonic cells of selected microorganisms. This method was optimized and subsequently applied to biofilm samples prepared on stainless steel coupons. The study results indicate that fluorescence in

Druh

C - Kapitola v odborné knize

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Laboratory Techniques with Applicability in Medical Practise

ISBN

978-3-659-31724-8

Počet stran výsledku

13

Strana od-do

33-45

Počet stran knihy

159

Název nakladatele

LAP Lambert Academic Publishing AG &amp; Co. KG

Místo vydání

Saarbrücken

Kód UT WoS kapitoly

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