The Effects of the Column Length on the Efficiency of Capillary Zwitterionic Organic Polymer Monolithic Columns in HILIC Chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900316" target="_blank" >RIV/00216275:25310/15:39900316 - isvavai.cz</a>

Výsledek na webu

<a href="http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=3&SID=U2wX1qdelgCSs7OzriD&page=1&doc=1" target="_blank" >http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=3&SID=U2wX1qdelgCSs7OzriD&page=1&doc=1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10337-015-2848-4" target="_blank" >10.1007/s10337-015-2848-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Effects of the Column Length on the Efficiency of Capillary Zwitterionic Organic Polymer Monolithic Columns in HILIC Chromatography

Popis výsledku v původním jazyce

Organic polymer monolithic columns of different lengths have been prepared in 320 A mu m i.d. fused silica capillary by in situ radical polymerization of N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl) ammonium betaine as a zwitterionic functional monomer and bisphenol A glycerolate dimethacrylate as a crosslinking monomer in the presence of porogenic solvents. The zwitterionic monolithic columns are intended for separations of polar compounds in hydrophilic interaction chromatography (HILIC). The effects of the capillary column length, from 115 to 175 mm, on separation efficiency, were investigated under HILIC conditions, using 95:5 acetonitrile in water as the mobile phase. The extra-column contributions to band broadening significantly decrease the efficiency (apparent height equivalent to a theoretical plate), especially for weakly retained samples, and increase with diminishing column length. The experimental height equivalents of theoretical plate, HETP, were corrected for the

Název v anglickém jazyce

The Effects of the Column Length on the Efficiency of Capillary Zwitterionic Organic Polymer Monolithic Columns in HILIC Chromatography

Popis výsledku anglicky

Organic polymer monolithic columns of different lengths have been prepared in 320 A mu m i.d. fused silica capillary by in situ radical polymerization of N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl) ammonium betaine as a zwitterionic functional monomer and bisphenol A glycerolate dimethacrylate as a crosslinking monomer in the presence of porogenic solvents. The zwitterionic monolithic columns are intended for separations of polar compounds in hydrophilic interaction chromatography (HILIC). The effects of the capillary column length, from 115 to 175 mm, on separation efficiency, were investigated under HILIC conditions, using 95:5 acetonitrile in water as the mobile phase. The extra-column contributions to band broadening significantly decrease the efficiency (apparent height equivalent to a theoretical plate), especially for weakly retained samples, and increase with diminishing column length. The experimental height equivalents of theoretical plate, HETP, were corrected for the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GAP206%2F12%2F0398" target="_blank" >GAP206/12/0398: Dvourozměrné separace v kapalných médiích</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chromatographia

ISSN

0009-5893

e-ISSN

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Svazek periodika

79

Číslo periodika v rámci svazku

13-14

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

853-859

Kód UT WoS článku

000357460300002

EID výsledku v databázi Scopus

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