Separation of flavonoids on different phenyl-bonded stationary phases-the influence of polar groups in stationary phase structure

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F16%3A39901384" target="_blank" >RIV/00216275:25310/16:39901384 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2015.12.024" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2015.12.024</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2015.12.024" target="_blank" >10.1016/j.chroma.2015.12.024</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Separation of flavonoids on different phenyl-bonded stationary phases-the influence of polar groups in stationary phase structure

Popis výsledku v původním jazyce

Four phenyl-bonded stationary phases, differing in polar embedded group between spacer and phenyl ring, were used for the separation of flavonoids in reversed-phase conditions. In addition, the work was focused on the comparison of these stationary phases in terms of retention and nature of interactions between flavonoid solutes and both, mobile and stationary phases. The differences and similarities between the columns and between individual flavonoids were evaluated by a statistical analysis. The retention over the wider range of mobile phase composition was described using well known model suggested for partition chromatographic systems. Due to differences in polarity of flavonoids, gradient elution had to be applied to achieve appropriate conditions for the successful separation. A chromatographic optimization software was employed for establish the appropriate profiles of gradient separations using UV detection at 275 nm. The most appropriate conditions for the separation of flavonoids were apparent on the phenyl and phenoxy columns.

Název v anglickém jazyce

Separation of flavonoids on different phenyl-bonded stationary phases-the influence of polar groups in stationary phase structure

Popis výsledku anglicky

Four phenyl-bonded stationary phases, differing in polar embedded group between spacer and phenyl ring, were used for the separation of flavonoids in reversed-phase conditions. In addition, the work was focused on the comparison of these stationary phases in terms of retention and nature of interactions between flavonoid solutes and both, mobile and stationary phases. The differences and similarities between the columns and between individual flavonoids were evaluated by a statistical analysis. The retention over the wider range of mobile phase composition was described using well known model suggested for partition chromatographic systems. Due to differences in polarity of flavonoids, gradient elution had to be applied to achieve appropriate conditions for the successful separation. A chromatographic optimization software was employed for establish the appropriate profiles of gradient separations using UV detection at 275 nm. The most appropriate conditions for the separation of flavonoids were apparent on the phenyl and phenoxy columns.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1429

Číslo periodika v rámci svazku

prosinec

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

198-206

Kód UT WoS článku

000369559100022

EID výsledku v databázi Scopus

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