Extraction, separation and identification of bioactive compounds in barley using LC-MS/MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F16%3A39901783" target="_blank" >RIV/00216275:25310/16:39901783 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Extraction, separation and identification of bioactive compounds in barley using LC-MS/MS

Popis výsledku v původním jazyce

An extraction procedure, based on alkaline and acidic hydrolysis, was developed, using the Box-Behnken design of experiment, with the aim to achieve an exhaustive recovery of free and also linked polyphenols, i.e. bonded to cell structures, in barley (Hordeum Vulgare L.) samples. An LC-MS/MS (MRM transition mode) method was developed for analysis of the samples extracted through the optimized procedure, and to achieve a correct quali-quantitative characterization of a specific group of polyphenols. The optimized procedure was also employed for the extraction and the identification of bioactive molecules in a dietary supplement composed of dried leaves of barley, commonly known as "young barley". A selectivity study was carried out employing some of the compounds identified in young barley, and by using six different columns (C18, silica, ES-CN and OH5 types). The results showed that different mechanisms are involved in the retention of the standards, especially in case of HILIC mode.

Název v anglickém jazyce

Extraction, separation and identification of bioactive compounds in barley using LC-MS/MS

Popis výsledku anglicky

An extraction procedure, based on alkaline and acidic hydrolysis, was developed, using the Box-Behnken design of experiment, with the aim to achieve an exhaustive recovery of free and also linked polyphenols, i.e. bonded to cell structures, in barley (Hordeum Vulgare L.) samples. An LC-MS/MS (MRM transition mode) method was developed for analysis of the samples extracted through the optimized procedure, and to achieve a correct quali-quantitative characterization of a specific group of polyphenols. The optimized procedure was also employed for the extraction and the identification of bioactive molecules in a dietary supplement composed of dried leaves of barley, commonly known as "young barley". A selectivity study was carried out employing some of the compounds identified in young barley, and by using six different columns (C18, silica, ES-CN and OH5 types). The results showed that different mechanisms are involved in the retention of the standards, especially in case of HILIC mode.

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

CECE 2016 : 13th International Interdisciplinary Meeting on Bioanalysis

ISBN

978-80-904959-4-4

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

59-62

Název nakladatele

Ústav analytické chemie AV ČR, v. v. i.

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

17. 10. 2016

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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