Retention behavior of lipids in reversed-phase ultrahigh-performanceliquid chromatography-electrospray ionization mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F16%3A39901790" target="_blank" >RIV/00216275:25310/16:39901790 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2016.04.082" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2016.04.082</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2016.04.082" target="_blank" >10.1016/j.chroma.2016.04.082</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Retention behavior of lipids in reversed-phase ultrahigh-performanceliquid chromatography-electrospray ionization mass spectrometry

Popis výsledku v původním jazyce

Reversed-phase ultrahigh-performance liquid chromatography (RP-UHPLC) method using two 15 cmsub-2 m particles octadecylsilica gel columns is developed with the goal to separate and unambigu-ously identify a large number of lipid species in biological samples. The identification is performed by thecoupling with high-resolution tandem mass spectrometry (MS/MS) using quadrupole - time-of-flight(QTOF) instrument. Electrospray ionization (ESI) full scan and tandem mass spectra are measured inboth polarity modes with the mass accuracy better than 5 ppm, which provides a high confidence oflipid identification. Over 400 lipid species covering 14 polar and nonpolar lipid classes from 5 lipid cate-gories are identified in total lipid extracts of human plasma, human urine and porcine brain. The generaldependences of relative retention times on relative carbon number or relative double bond number areconstructed and fit with the second degree polynomial regression. The regular retention patterns inhomologous lipid series provide additional identification point for UHPLC/MS lipidomic analysis, whichincreases the confidence of lipid identification. The reprocessing of previously published data by our andother groups measured in the RP mode and ultrahigh-performance supercritical fluid chromatographyon the silica column shows more generic applicability of the polynomial regression for the description ofretention behavior and the prediction of retention times. The novelty of this work is the characterizationof general trends in the retention behavior of lipids within logical series with constant fatty acyl lengthor double bond number, which may be used as an additional criterion to increase the confidence of lipididentification.

Název v anglickém jazyce

Retention behavior of lipids in reversed-phase ultrahigh-performanceliquid chromatography-electrospray ionization mass spectrometry

Popis výsledku anglicky

Reversed-phase ultrahigh-performance liquid chromatography (RP-UHPLC) method using two 15 cmsub-2 m particles octadecylsilica gel columns is developed with the goal to separate and unambigu-ously identify a large number of lipid species in biological samples. The identification is performed by thecoupling with high-resolution tandem mass spectrometry (MS/MS) using quadrupole - time-of-flight(QTOF) instrument. Electrospray ionization (ESI) full scan and tandem mass spectra are measured inboth polarity modes with the mass accuracy better than 5 ppm, which provides a high confidence oflipid identification. Over 400 lipid species covering 14 polar and nonpolar lipid classes from 5 lipid cate-gories are identified in total lipid extracts of human plasma, human urine and porcine brain. The generaldependences of relative retention times on relative carbon number or relative double bond number areconstructed and fit with the second degree polynomial regression. The regular retention patterns inhomologous lipid series provide additional identification point for UHPLC/MS lipidomic analysis, whichincreases the confidence of lipid identification. The reprocessing of previously published data by our andother groups measured in the RP mode and ultrahigh-performance supercritical fluid chromatographyon the silica column shows more generic applicability of the polynomial regression for the description ofretention behavior and the prediction of retention times. The novelty of this work is the characterizationof general trends in the retention behavior of lipids within logical series with constant fatty acyl lengthor double bond number, which may be used as an additional criterion to increase the confidence of lipididentification.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

<a href="/cs/project/LL1302" target="_blank" >LL1302: Hmotnostní spektrometrie při hledání lipidových biomarkerů pro včasnou diagnostiku rakoviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1450

Číslo periodika v rámci svazku

June

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

"76?85"

Kód UT WoS článku

000377323700009

EID výsledku v databázi Scopus

2-s2.0-84968750673