Determination of distribution constants of antioxidants by electrokinetic chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39910645" target="_blank" >RIV/00216275:25310/17:39910645 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/23312009.2017.1385173" target="_blank" >http://dx.doi.org/10.1080/23312009.2017.1385173</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/23312009.2017.1385173" target="_blank" >10.1080/23312009.2017.1385173</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of distribution constants of antioxidants by electrokinetic chromatography

Popis výsledku v původním jazyce

Liposome electrokinetic chromatography and micellar electrokinetic chromatography were used for studying the lipophilic properties of natural antioxidants, specifically phenolic acids and flavonoids. The employed negatively charged liposomes were composed of mixtures of 1-palmitoyl-2-oleyl-sn-glycerophosphati-dylcholine and 1-palmitoyl-2-oleoyl-sn-phosphatidylserine or 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol. In micellar electrokinetic chromatography, sodium dodecyl sulphate micelles were used as the pseudostationary phase. The retention factors of the studied compounds were determined at pH 7.4. The corresponding distribution constants were calculated from the experimentally determined retention factors and the phase ratios of the liposome dispersions and the micellar system. The distribution constants between the aqueous phase and the liposomes or micelles were compared with octanol/water partition or distribution constants of the studied compounds, which were predicted using the ACD/Labs Percepta Platform-PhysChem Module. Our results indicate that the correlations between the distribution constants of the two tested liposome systems were much stronger than those between the liposome/micellar systems. The correlations between the n-octanol phase and the liposome phases were similar to that between n-octanol and the micellar phase. Our data shows that electrokinetic chromatography is an efficient method for determining partition coefficients of compounds, but the type of pseudostationary phase has a clear impact on the values.

Název v anglickém jazyce

Determination of distribution constants of antioxidants by electrokinetic chromatography

Popis výsledku anglicky

Liposome electrokinetic chromatography and micellar electrokinetic chromatography were used for studying the lipophilic properties of natural antioxidants, specifically phenolic acids and flavonoids. The employed negatively charged liposomes were composed of mixtures of 1-palmitoyl-2-oleyl-sn-glycerophosphati-dylcholine and 1-palmitoyl-2-oleoyl-sn-phosphatidylserine or 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol. In micellar electrokinetic chromatography, sodium dodecyl sulphate micelles were used as the pseudostationary phase. The retention factors of the studied compounds were determined at pH 7.4. The corresponding distribution constants were calculated from the experimentally determined retention factors and the phase ratios of the liposome dispersions and the micellar system. The distribution constants between the aqueous phase and the liposomes or micelles were compared with octanol/water partition or distribution constants of the studied compounds, which were predicted using the ACD/Labs Percepta Platform-PhysChem Module. Our results indicate that the correlations between the distribution constants of the two tested liposome systems were much stronger than those between the liposome/micellar systems. The correlations between the n-octanol phase and the liposome phases were similar to that between n-octanol and the micellar phase. Our data shows that electrokinetic chromatography is an efficient method for determining partition coefficients of compounds, but the type of pseudostationary phase has a clear impact on the values.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

COGENT CHEMISTRY

ISSN

2331-2009

e-ISSN

—

Svazek periodika

3

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NO - Norské království

Počet stran výsledku

11

Strana od-do

"1385173-1"-"1385173-11"

Kód UT WoS článku

000412345300001

EID výsledku v databázi Scopus

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