Monolithic Capillary Column with an Integrated Electrochemical Detector

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39911330" target="_blank" >RIV/00216275:25310/17:39911330 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/17:00113892

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0021967317309251" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0021967317309251</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2017.06.057" target="_blank" >10.1016/j.chroma.2017.06.057</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Monolithic Capillary Column with an Integrated Electrochemical Detector

Popis výsledku v původním jazyce

The carbon fiber and silver microwire were used as working and pseudoreference electrode, respectively, and inserted into the ending of capillary to prepare monolithic capillary column with an integrated electrochemical detector. Prepared capillary devices offered stable and robust results with relative standard deviations of retention, resolution, and detection signal lower than 1.5, 5.5, and 5.0%, respectively. To further increase sensitivity of developed electrochemical microdetector, multiple pulse amperometry detection mode has been used. Optimized integrated device provided reliable chromatographic separation of mixture of neurotransmitters with calibration curve for dopamine linear from 0.5 to 20.0 mg L-1 and an instrumental limit of detection as low as 24 pg of injected dopamine. Finally, developed capillary column was applied to successful determination of dopamine in a human urine. By using both calibration curve and standard addition method, the dopamine level was determined to be 0.74 +/- 0.03 mgL(-1) and 0.71 +/- 0.02 mgL(-1), respectively. Triplicates of dopamine analysis provided relative standard deviations lower than 2.7% for intraday analyses, while interday relative standard deviations were lower than 3.6% for five consecutive days.

Název v anglickém jazyce

Monolithic Capillary Column with an Integrated Electrochemical Detector

Popis výsledku anglicky

The carbon fiber and silver microwire were used as working and pseudoreference electrode, respectively, and inserted into the ending of capillary to prepare monolithic capillary column with an integrated electrochemical detector. Prepared capillary devices offered stable and robust results with relative standard deviations of retention, resolution, and detection signal lower than 1.5, 5.5, and 5.0%, respectively. To further increase sensitivity of developed electrochemical microdetector, multiple pulse amperometry detection mode has been used. Optimized integrated device provided reliable chromatographic separation of mixture of neurotransmitters with calibration curve for dopamine linear from 0.5 to 20.0 mg L-1 and an instrumental limit of detection as low as 24 pg of injected dopamine. Finally, developed capillary column was applied to successful determination of dopamine in a human urine. By using both calibration curve and standard addition method, the dopamine level was determined to be 0.74 +/- 0.03 mgL(-1) and 0.71 +/- 0.02 mgL(-1), respectively. Triplicates of dopamine analysis provided relative standard deviations lower than 2.7% for intraday analyses, while interday relative standard deviations were lower than 3.6% for five consecutive days.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA14-22426S" target="_blank" >GA14-22426S: Vývoj multifunkční monolitické kapilární kolony s integrovanou úpravou vzorku, separací a elektrochemickou detekcí</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1509

Číslo periodika v rámci svazku

August

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

171-175

Kód UT WoS článku

000407868800020

EID výsledku v databázi Scopus

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