New Interface for Purification of Proteins: One-Dimensional TiO2 Nanotubes Decorated by Fe3O4 Nanoparticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39911337" target="_blank" >RIV/00216275:25310/17:39911337 - isvavai.cz</a>

Výsledek na webu

<a href="http://pubs.acs.org/doi/abs/10.1021/acsami.7b08445" target="_blank" >http://pubs.acs.org/doi/abs/10.1021/acsami.7b08445</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsami.7b08445" target="_blank" >10.1021/acsami.7b08445</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New Interface for Purification of Proteins: One-Dimensional TiO2 Nanotubes Decorated by Fe3O4 Nanoparticles

Popis výsledku v původním jazyce

In this work, a high surface area interface, based on anodic one-dimensional (1D) TiO2 nanotubes homogeneously decorated by Fe3O4 nanoparticles (TiO2NTs@Fe3O4NPs is reported for the first time for an unprecedented purification of His-tagged recombinant proteins. Excellent purification results were achieved from the model protein mixture, as well as from the whole cell lysate (with His -tagged ubiquitin). Compared to a conventional immobilized -metal affinity chromatography (IMAC) system, specific isolation of selected His -tagged proteins on behalf of other proteins was significantly enhanced on TiO2NTs@Fe3O4NPs interface under optimized binding and elution conditions. The combination of specific isolation properties, magnetic features, biocompatibility, and ease of preparation of this material consisting of two basic metal oxides makes it a suitable candidate for future purification of recombinant proteins in biotechnology. The principally new: material bears a large potential to open new pathways for discoveries in nanobiotechnology and nanomedicine.

Název v anglickém jazyce

New Interface for Purification of Proteins: One-Dimensional TiO2 Nanotubes Decorated by Fe3O4 Nanoparticles

Popis výsledku anglicky

In this work, a high surface area interface, based on anodic one-dimensional (1D) TiO2 nanotubes homogeneously decorated by Fe3O4 nanoparticles (TiO2NTs@Fe3O4NPs is reported for the first time for an unprecedented purification of His-tagged recombinant proteins. Excellent purification results were achieved from the model protein mixture, as well as from the whole cell lysate (with His -tagged ubiquitin). Compared to a conventional immobilized -metal affinity chromatography (IMAC) system, specific isolation of selected His -tagged proteins on behalf of other proteins was significantly enhanced on TiO2NTs@Fe3O4NPs interface under optimized binding and elution conditions. The combination of specific isolation properties, magnetic features, biocompatibility, and ease of preparation of this material consisting of two basic metal oxides makes it a suitable candidate for future purification of recombinant proteins in biotechnology. The principally new: material bears a large potential to open new pathways for discoveries in nanobiotechnology and nanomedicine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Applied Materials &amp; Interfaces

ISSN

1944-8244

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

34

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

"28233–28242"

Kód UT WoS článku

000409395500012

EID výsledku v databázi Scopus

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