Magnetic macroporous bead cellulose functionalised with recombinant hyaluronan lyase for controllable hyaluronan fragmentation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F18%3A39913177" target="_blank" >RIV/00216275:25310/18:39913177 - isvavai.cz</a>

Výsledek na webu

<a href="http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=6&SID=C2pzRncJPo1FecCVZHU&page=1&doc=1" target="_blank" >http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=6&SID=C2pzRncJPo1FecCVZHU&page=1&doc=1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.procbio.2018.06.025" target="_blank" >10.1016/j.procbio.2018.06.025</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Magnetic macroporous bead cellulose functionalised with recombinant hyaluronan lyase for controllable hyaluronan fragmentation

Popis výsledku v původním jazyce

Hyaluronic acid (HA) is a component of the extracellular matrix with important potential for biotechnological and pharmaceutical applications. However, the immunological effects of HA are strongly molecular weight-dependent; therefore, production of HA for therapeutic purposes requires control over the molecular weight of the product, as well as mild reaction conditions and elimination of bioactive impurities. Here, we produced and characterised biocompatible magnetic macroporous bead cellulose (MBC) functionalised with Streptococcus pneumoniae hyaluronan lyase (SpnHL) that are compatible with these requirements and optimised their reaction and storage conditions. Immobilisation of SpnHL on MBC via reductive amination or MBC with fixed iminodiacetic acid (MBC-IDA) via a His(8)-tag had minimal impact on its catalytic activity. The MBC-IDA-SpnHL carrier showed excellent operational and storage stability, and both carriers enabled reproducible time-controlled fragmentation of highly viscous HMW HA solutions, yielding HA fragments of appropriate molecular weight. Thus, these carriers are suitable for numerous industrial and biotechnological applications.

Název v anglickém jazyce

Magnetic macroporous bead cellulose functionalised with recombinant hyaluronan lyase for controllable hyaluronan fragmentation

Popis výsledku anglicky

Hyaluronic acid (HA) is a component of the extracellular matrix with important potential for biotechnological and pharmaceutical applications. However, the immunological effects of HA are strongly molecular weight-dependent; therefore, production of HA for therapeutic purposes requires control over the molecular weight of the product, as well as mild reaction conditions and elimination of bioactive impurities. Here, we produced and characterised biocompatible magnetic macroporous bead cellulose (MBC) functionalised with Streptococcus pneumoniae hyaluronan lyase (SpnHL) that are compatible with these requirements and optimised their reaction and storage conditions. Immobilisation of SpnHL on MBC via reductive amination or MBC with fixed iminodiacetic acid (MBC-IDA) via a His(8)-tag had minimal impact on its catalytic activity. The MBC-IDA-SpnHL carrier showed excellent operational and storage stability, and both carriers enabled reproducible time-controlled fragmentation of highly viscous HMW HA solutions, yielding HA fragments of appropriate molecular weight. Thus, these carriers are suitable for numerous industrial and biotechnological applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Process biochemistry

ISSN

1359-5113

e-ISSN

—

Svazek periodika

72

Číslo periodika v rámci svazku

72

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

105-111

Kód UT WoS článku

000442710600013

EID výsledku v databázi Scopus

2-s2.0-85049564986