A porcine model of skin wound infected with a polybacterial biofilm

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F18%3A39914242" target="_blank" >RIV/00216275:25310/18:39914242 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/18:00495966 RIV/00216208:11120/18:43916259 RIV/00216208:11140/18:10369468 RIV/00216208:11150/18:10369468 a 2 dalších

Výsledek na webu

<a href="https://www.tandfonline.com/doi/abs/10.1080/08927014.2018.1425684" target="_blank" >https://www.tandfonline.com/doi/abs/10.1080/08927014.2018.1425684</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/08927014.2018.1425684" target="_blank" >10.1080/08927014.2018.1425684</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A porcine model of skin wound infected with a polybacterial biofilm

Popis výsledku v původním jazyce

A clinically relevant porcine model of a biofilm-infected wound was established in 10 minipigs. The wounds of six experimental animals were infected with a modified polymicrobial Lubbock chronic wound biofilm consisting of Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Bacillus subtilis. Four animals served as uninfected controls. The wounds were monitored until they had healed for 24 days. The biofilm persisted in the wounds up to day 14 and significantly affected healing. The control to infected healed wound area ratios were: 45%/21%, 66%/37%, and 90%/57% on days 7, 10 and 14, respectively. The implanted biofilm prolonged inflammation, increased necrosis, delayed granulation and impaired development of the extracellular matrix as seen in histological and gene expression analyses. This model provides a therapeutic one-week window for testing of anti-biofilm treatments and for research on the pathogenesis of wound infections in pig that is clinically the most relevant animal wound healing model.

Název v anglickém jazyce

A porcine model of skin wound infected with a polybacterial biofilm

Popis výsledku anglicky

A clinically relevant porcine model of a biofilm-infected wound was established in 10 minipigs. The wounds of six experimental animals were infected with a modified polymicrobial Lubbock chronic wound biofilm consisting of Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Bacillus subtilis. Four animals served as uninfected controls. The wounds were monitored until they had healed for 24 days. The biofilm persisted in the wounds up to day 14 and significantly affected healing. The control to infected healed wound area ratios were: 45%/21%, 66%/37%, and 90%/57% on days 7, 10 and 14, respectively. The implanted biofilm prolonged inflammation, increased necrosis, delayed granulation and impaired development of the extracellular matrix as seen in histological and gene expression analyses. This model provides a therapeutic one-week window for testing of anti-biofilm treatments and for research on the pathogenesis of wound infections in pig that is clinically the most relevant animal wound healing model.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20801 - Environmental biotechnology

Projekt

<a href="/cs/project/TA03011029" target="_blank" >TA03011029: Nové kryty ran s programovaným uvolňováním účinných látek určené pro inhibici biofilmu.</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biofouling

ISSN

0892-7014

e-ISSN

—

Svazek periodika

34

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

226-236

Kód UT WoS článku

000424418500010

EID výsledku v databázi Scopus

2-s2.0-85041405109