Liquid Chromatography | Multidimensional
Popis výsledku
Identifikátory výsledku
Kód výsledku v IS VaVaI
Výsledek na webu
https://www.sciencedirect.com/science/article/pii/B9780124095472144944?via%3Dihub
DOI - Digital Object Identifier
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Liquid Chromatography | Multidimensional
Popis výsledku v původním jazyce
In clinical, pharmaceutical, food, natural and environmental analysis, molecular biology and elsewhere, the samples may contain even millions of compounds, in concentrations possibly spanning over 10 orders of magnitude. The maximum theoretical number of peaks that we can separate in a single run HPLC hardly can exceed 100. Combining two or more different separation columns, the theoretical peak capacity, that is, the maximum number of peaks separated in a fixed time can be significantly increased. A chromatographic column together with the operating conditions affecting the retention and selectivity of separation, such as mobile phase, temperature, pH, gradient, etc., represent the separation medium. In one-dimensional separation systems, a single separation medium is used. A system consisting of several separation media is multidimensional (MD), when the separation mechanisms in each dimension are different With any additional separation dimension, the number of resolved compounds, but also the experimental complexity of the MD system and the time requirements dramatically increase. So far, two-dimensional liquid chromatography (2D LC) systems are used in practice; three-dimensional (3D LC) systems are investigated. In addition to major increase in peak capacity, multidimensional separations may provide group separations of various samples based on molecular structure similarities and differences.
Název v anglickém jazyce
Liquid Chromatography | Multidimensional
Popis výsledku anglicky
In clinical, pharmaceutical, food, natural and environmental analysis, molecular biology and elsewhere, the samples may contain even millions of compounds, in concentrations possibly spanning over 10 orders of magnitude. The maximum theoretical number of peaks that we can separate in a single run HPLC hardly can exceed 100. Combining two or more different separation columns, the theoretical peak capacity, that is, the maximum number of peaks separated in a fixed time can be significantly increased. A chromatographic column together with the operating conditions affecting the retention and selectivity of separation, such as mobile phase, temperature, pH, gradient, etc., represent the separation medium. In one-dimensional separation systems, a single separation medium is used. A system consisting of several separation media is multidimensional (MD), when the separation mechanisms in each dimension are different With any additional separation dimension, the number of resolved compounds, but also the experimental complexity of the MD system and the time requirements dramatically increase. So far, two-dimensional liquid chromatography (2D LC) systems are used in practice; three-dimensional (3D LC) systems are investigated. In addition to major increase in peak capacity, multidimensional separations may provide group separations of various samples based on molecular structure similarities and differences.
Klasifikace
Druh
C - Kapitola v odborné knize
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název knihy nebo sborníku
Encyclopedia of Analytical Science
ISBN
978-0-08-101983-2
Počet stran výsledku
8
Strana od-do
154-161
Počet stran knihy
5109
Název nakladatele
Elsevier Science BV
Místo vydání
Amsterdam
Kód UT WoS kapitoly
—
Základní informace
Druh výsledku
C - Kapitola v odborné knize
OECD FORD
Analytical chemistry
Rok uplatnění
2019