Liquid Chromatography | Multidimensional

Kód výsledku v IS VaVaI

RIV/00216275:25310/19:39914819 - isvavai.cz

Výsledek na webu

https://www.sciencedirect.com/science/article/pii/B9780124095472144944?via%3Dihub

DOI - Digital Object Identifier

10.1016/B978-0-12-409547-2.14494-4

Jazyk výsledku

angličtina

Název v původním jazyce

Liquid Chromatography | Multidimensional

Popis výsledku v původním jazyce

In clinical, pharmaceutical, food, natural and environmental analysis, molecular biology and elsewhere, the samples may contain even millions of compounds, in concentrations possibly spanning over 10 orders of magnitude. The maximum theoretical number of peaks that we can separate in a single run HPLC hardly can exceed 100. Combining two or more different separation columns, the theoretical peak capacity, that is, the maximum number of peaks separated in a fixed time can be significantly increased. A chromatographic column together with the operating conditions affecting the retention and selectivity of separation, such as mobile phase, temperature, pH, gradient, etc., represent the separation medium. In one-dimensional separation systems, a single separation medium is used. A system consisting of several separation media is multidimensional (MD), when the separation mechanisms in each dimension are different With any additional separation dimension, the number of resolved compounds, but also the experimental complexity of the MD system and the time requirements dramatically increase. So far, two-dimensional liquid chromatography (2D LC) systems are used in practice; three-dimensional (3D LC) systems are investigated. In addition to major increase in peak capacity, multidimensional separations may provide group separations of various samples based on molecular structure similarities and differences.

Název v anglickém jazyce

Liquid Chromatography | Multidimensional

Popis výsledku anglicky

In clinical, pharmaceutical, food, natural and environmental analysis, molecular biology and elsewhere, the samples may contain even millions of compounds, in concentrations possibly spanning over 10 orders of magnitude. The maximum theoretical number of peaks that we can separate in a single run HPLC hardly can exceed 100. Combining two or more different separation columns, the theoretical peak capacity, that is, the maximum number of peaks separated in a fixed time can be significantly increased. A chromatographic column together with the operating conditions affecting the retention and selectivity of separation, such as mobile phase, temperature, pH, gradient, etc., represent the separation medium. In one-dimensional separation systems, a single separation medium is used. A system consisting of several separation media is multidimensional (MD), when the separation mechanisms in each dimension are different With any additional separation dimension, the number of resolved compounds, but also the experimental complexity of the MD system and the time requirements dramatically increase. So far, two-dimensional liquid chromatography (2D LC) systems are used in practice; three-dimensional (3D LC) systems are investigated. In addition to major increase in peak capacity, multidimensional separations may provide group separations of various samples based on molecular structure similarities and differences.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Encyclopedia of Analytical Science

ISBN

978-0-08-101983-2

Počet stran výsledku

8

Strana od-do

154-161

Počet stran knihy

5109

Název nakladatele

Elsevier Science BV

Místo vydání

Amsterdam

Kód UT WoS kapitoly

—