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Liquid Chromatography | Multidimensional

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Liquid Chromatography | Multidimensional

  • Popis výsledku v původním jazyce

    In clinical, pharmaceutical, food, natural and environmental analysis, molecular biology and elsewhere, the samples may contain even millions of compounds, in concentrations possibly spanning over 10 orders of magnitude. The maximum theoretical number of peaks that we can separate in a single run HPLC hardly can exceed 100. Combining two or more different separation columns, the theoretical peak capacity, that is, the maximum number of peaks separated in a fixed time can be significantly increased. A chromatographic column together with the operating conditions affecting the retention and selectivity of separation, such as mobile phase, temperature, pH, gradient, etc., represent the separation medium. In one-dimensional separation systems, a single separation medium is used. A system consisting of several separation media is multidimensional (MD), when the separation mechanisms in each dimension are different With any additional separation dimension, the number of resolved compounds, but also the experimental complexity of the MD system and the time requirements dramatically increase. So far, two-dimensional liquid chromatography (2D LC) systems are used in practice; three-dimensional (3D LC) systems are investigated. In addition to major increase in peak capacity, multidimensional separations may provide group separations of various samples based on molecular structure similarities and differences.

  • Název v anglickém jazyce

    Liquid Chromatography | Multidimensional

  • Popis výsledku anglicky

    In clinical, pharmaceutical, food, natural and environmental analysis, molecular biology and elsewhere, the samples may contain even millions of compounds, in concentrations possibly spanning over 10 orders of magnitude. The maximum theoretical number of peaks that we can separate in a single run HPLC hardly can exceed 100. Combining two or more different separation columns, the theoretical peak capacity, that is, the maximum number of peaks separated in a fixed time can be significantly increased. A chromatographic column together with the operating conditions affecting the retention and selectivity of separation, such as mobile phase, temperature, pH, gradient, etc., represent the separation medium. In one-dimensional separation systems, a single separation medium is used. A system consisting of several separation media is multidimensional (MD), when the separation mechanisms in each dimension are different With any additional separation dimension, the number of resolved compounds, but also the experimental complexity of the MD system and the time requirements dramatically increase. So far, two-dimensional liquid chromatography (2D LC) systems are used in practice; three-dimensional (3D LC) systems are investigated. In addition to major increase in peak capacity, multidimensional separations may provide group separations of various samples based on molecular structure similarities and differences.

Klasifikace

  • Druh

    C - Kapitola v odborné knize

  • CEP obor

  • OECD FORD obor

    10406 - Analytical chemistry

Návaznosti výsledku

  • Projekt

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2019

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název knihy nebo sborníku

    Encyclopedia of Analytical Science

  • ISBN

    978-0-08-101983-2

  • Počet stran výsledku

    8

  • Strana od-do

    154-161

  • Počet stran knihy

    5109

  • Název nakladatele

    Elsevier Science BV

  • Místo vydání

    Amsterdam

  • Kód UT WoS kapitoly