Multichannel separation device with parallel electrochemical detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F20%3A39916575" target="_blank" >RIV/00216275:25310/20:39916575 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/20:00114497

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0021967319309306" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0021967319309306</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2019.460537" target="_blank" >10.1016/j.chroma.2019.460537</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multichannel separation device with parallel electrochemical detection

Popis výsledku v původním jazyce

A device with four parallel channels was designed and manufactured by 3D printing in titanium. A simple experimental setup allowed splitting of the mobile phase in four parallel streams, such that a single sample could be analysed four times simultaneously. The four capillary channels were filled with a monolithic stationary phase, prepared using a zwitterionic functional monomer in combination with various dimethacrylate cross-linkers. The resulting stationary phases were applicable in both reversed-phase and hydrophilic-interaction retention mechanisms. The mobile-phase composition was optimized by means of a window diagram so as to obtain the highest possible resolution of dopamine precursors and metabolites on all columns. Miniaturized electrochemical detectors with carbon fibres as working electrodes and silver micro-wires as reference electrodes were integrated in the device at the end of each column. Experimental separations were successfully compared with those predicted by a three-parameter retention model. Finally, dopamine was determined in human urine to further confirm applicability of the developed device. (C) 2019 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Multichannel separation device with parallel electrochemical detection

Popis výsledku anglicky

A device with four parallel channels was designed and manufactured by 3D printing in titanium. A simple experimental setup allowed splitting of the mobile phase in four parallel streams, such that a single sample could be analysed four times simultaneously. The four capillary channels were filled with a monolithic stationary phase, prepared using a zwitterionic functional monomer in combination with various dimethacrylate cross-linkers. The resulting stationary phases were applicable in both reversed-phase and hydrophilic-interaction retention mechanisms. The mobile-phase composition was optimized by means of a window diagram so as to obtain the highest possible resolution of dopamine precursors and metabolites on all columns. Miniaturized electrochemical detectors with carbon fibres as working electrodes and silver micro-wires as reference electrodes were integrated in the device at the end of each column. Experimental separations were successfully compared with those predicted by a three-parameter retention model. Finally, dopamine was determined in human urine to further confirm applicability of the developed device. (C) 2019 Elsevier B.V. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA17-11252S" target="_blank" >GA17-11252S: Polymerní monolitické stacionární fáze s kontrolovanou selektivitou</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1610

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

460537

Kód UT WoS článku

000509816200013

EID výsledku v databázi Scopus

2-s2.0-85072163849