Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F21%3A39917818" target="_blank" >RIV/00216275:25310/21:39917818 - isvavai.cz</a>

Výsledek na webu

<a href="https://reader.elsevier.com/reader/sd/pii/S2211124721012572?token=DB0A0ACFE04FC8E3734585A75BDBD781AE2726A34534D990185FDEBD512DCEFF88D66E62104ABCE92A97C275D333DDE7&originRegion=eu-west-1&originCreation=20211214135115" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S2211124721012572?token=DB0A0ACFE04FC8E3734585A75BDBD781AE2726A34534D990185FDEBD512DCEFF88D66E62104ABCE92A97C275D333DDE7&originRegion=eu-west-1&originCreation=20211214135115</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.celrep.2021.109797" target="_blank" >10.1016/j.celrep.2021.109797</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment

Popis výsledku v původním jazyce

Membrane lipids and their metabolism have key functions in neurotransmission. Here we provide a quantitative lipid inventory of mouse and rat synaptic junctions. To this end, we developed a multiomics extraction and analysis workflow to probe the interplay of proteins and lipids in synaptic signal transduction from the same sample. Based on this workflow, we generate hypotheses about novel mechanisms underlying complex changes in synaptic connectivity elicited by environmental stimuli. As a proof of principle, this approach reveals that in mice exposed to an enriched environment, reduced endocannabinoid synthesis and signaling is linked to increased surface expression of a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) in a subset of Cannabinoid-receptor 1 positive synapses. This mechanism regulates synaptic strength in an input-specific manner. Thus, we establish a compartment-specific multiomics workflow that is suitable to extract information from complex lipid and protein networks involved in synaptic function and plasticity.

Název v anglickém jazyce

Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment

Popis výsledku anglicky

Membrane lipids and their metabolism have key functions in neurotransmission. Here we provide a quantitative lipid inventory of mouse and rat synaptic junctions. To this end, we developed a multiomics extraction and analysis workflow to probe the interplay of proteins and lipids in synaptic signal transduction from the same sample. Based on this workflow, we generate hypotheses about novel mechanisms underlying complex changes in synaptic connectivity elicited by environmental stimuli. As a proof of principle, this approach reveals that in mice exposed to an enriched environment, reduced endocannabinoid synthesis and signaling is linked to increased surface expression of a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) in a subset of Cannabinoid-receptor 1 positive synapses. This mechanism regulates synaptic strength in an input-specific manner. Thus, we establish a compartment-specific multiomics workflow that is suitable to extract information from complex lipid and protein networks involved in synaptic function and plasticity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/GA21-20238S" target="_blank" >GA21-20238S: Spojení vícerozměrné chromatografie a hmotnostní spektrometrie v kvantitativních přístupech pro detailní charakterizaci lipidomu lidské plazmy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell Reports

ISSN

2211-1247

e-ISSN

—

Svazek periodika

37

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

28

Strana od-do

109797

Kód UT WoS článku

000704665900025

EID výsledku v databázi Scopus

2-s2.0-85116421693