Ultrahigh-performance supercritical fluid chromatography / mass spectrometry in the lipidomic analysis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F22%3A39918849" target="_blank" >RIV/00216275:25310/22:39918849 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0165993622000292" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0165993622000292</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.trac.2022.116546" target="_blank" >10.1016/j.trac.2022.116546</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Ultrahigh-performance supercritical fluid chromatography / mass spectrometry in the lipidomic analysis
Popis výsledku v původním jazyce
Ultrahigh-performance supercritical fluid chromatography/mass spectrometry (UHPSFC/MS) is one of the fastest analytical approaches for high-throughput lipidomic analysis covering a wide range of lipid categories and classes. Lipid class separation and lipid species separation are two main separation modes available for the UHPSFC analysis of lipids. The mechanism of the lipid class separation mode is com-parable to that of hydrophilic interaction liquid chromatography (HILIC). The direct comparison of HILIC -ultrahigh-performance liquid chromatography (HILIC-UHPLC) and UHPSFC shows the applicability of UHPSFC/MS for high-throughput quantitation of both nonpolar and polar lipid classes, the faster analysis time, and comparable reproducibility and sensitivity. The retention mechanism of the lipid species separation mode is comparable to reversed-phase UHPLC, but the nonpolar character of supercritical carbon dioxide (scCO(2)) provides a different selectivity compared to that of conventional RP-UHPLC. The introduction of various hyphenation interfaces to MS broadens the applicability and sensitivity range of UHPSFC/MS using scCO(2) as the main component of the mobile phase. The addition of ionic additives (volatile salts, acids, and bases) to the modifier improves the separation of medium to polar lipids. Furthermore, UHPSFC/MS has also a great potential in the chiral separation of lipids, but the field has not yet been fully explored.
Název v anglickém jazyce
Ultrahigh-performance supercritical fluid chromatography / mass spectrometry in the lipidomic analysis
Popis výsledku anglicky
Ultrahigh-performance supercritical fluid chromatography/mass spectrometry (UHPSFC/MS) is one of the fastest analytical approaches for high-throughput lipidomic analysis covering a wide range of lipid categories and classes. Lipid class separation and lipid species separation are two main separation modes available for the UHPSFC analysis of lipids. The mechanism of the lipid class separation mode is com-parable to that of hydrophilic interaction liquid chromatography (HILIC). The direct comparison of HILIC -ultrahigh-performance liquid chromatography (HILIC-UHPLC) and UHPSFC shows the applicability of UHPSFC/MS for high-throughput quantitation of both nonpolar and polar lipid classes, the faster analysis time, and comparable reproducibility and sensitivity. The retention mechanism of the lipid species separation mode is comparable to reversed-phase UHPLC, but the nonpolar character of supercritical carbon dioxide (scCO(2)) provides a different selectivity compared to that of conventional RP-UHPLC. The introduction of various hyphenation interfaces to MS broadens the applicability and sensitivity range of UHPSFC/MS using scCO(2) as the main component of the mobile phase. The addition of ionic additives (volatile salts, acids, and bases) to the modifier improves the separation of medium to polar lipids. Furthermore, UHPSFC/MS has also a great potential in the chiral separation of lipids, but the field has not yet been fully explored.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/GA21-20238S" target="_blank" >GA21-20238S: Spojení vícerozměrné chromatografie a hmotnostní spektrometrie v kvantitativních přístupech pro detailní charakterizaci lipidomu lidské plazmy</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
TrAC - Trends in Analytical Chemistry
ISSN
0165-9936
e-ISSN
1879-3142
Svazek periodika
149
Číslo periodika v rámci svazku
April
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
10
Strana od-do
116546
Kód UT WoS článku
000790927700001
EID výsledku v databázi Scopus
2-s2.0-85124286290