TiO2 P25 nanoparticles induce mitochondrial damage and increased glutathione synthesis in SH-SY5Y neural cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F25%3A39923138" target="_blank" >RIV/00216275:25310/25:39923138 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.fct.2025.115496" target="_blank" >https://doi.org/10.1016/j.fct.2025.115496</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fct.2025.115496" target="_blank" >10.1016/j.fct.2025.115496</a>

Jazyk výsledku

angličtina

Název v původním jazyce

TiO2 P25 nanoparticles induce mitochondrial damage and increased glutathione synthesis in SH-SY5Y neural cells

Popis výsledku v původním jazyce

The increasing occurrence of TiO2 nanoparticles in the environment leads to a higher risk of their entry into the human body. Because the nanomaterials can cross the blood-brain barrier, the knowledge of their effect in neural cells is also crucial. However, the exact mechanism of toxicity remains unclear. Therefore, our objective was to evaluate the biological effect of TiO2 nanoparticles in neural cells. Therefore, we aimed to evaluate the biological effect of TiO2 nanoparticles in neural cells. We used neuroblastoma SH-SY5Y cells treated with 25 nm TiO2 nanoparticles (TiO2 P25; 1-100 µg.mL(-1); 24-72 h). We measured dehydrogenase activity, glutathione metabolism, DNA damage and mitochondrial respiration. After treatment of SH-SY5Y cells with TiO2 P25, we found a dose- and time-dependent decrease in dehydrogenase activity together with nuclear changes. Interestingly, 100 µg.mL(-1) TiO2 P25 caused a significant increase of glutathione levels (p &lt; 0.001) linked with increased glutamate-cysteine ligase expression after both 24 and 48 h. Furthermore, as the first study at all, we observed the significant decreases of mitochondrial respiration in SH-SY5Y cells caused by treatments with 10 and 100 µg.mL(-1) TiO2 P25 after 48 and 72 h. In conclusion, our study brings new finding of occurrence of decreased mitochondrial respiration without glutathione depletion in SH-SY5Y cells contributing to the understanding of TiO2 P25 toxicity in neural cells.

Název v anglickém jazyce

TiO2 P25 nanoparticles induce mitochondrial damage and increased glutathione synthesis in SH-SY5Y neural cells

Popis výsledku anglicky

The increasing occurrence of TiO2 nanoparticles in the environment leads to a higher risk of their entry into the human body. Because the nanomaterials can cross the blood-brain barrier, the knowledge of their effect in neural cells is also crucial. However, the exact mechanism of toxicity remains unclear. Therefore, our objective was to evaluate the biological effect of TiO2 nanoparticles in neural cells. Therefore, we aimed to evaluate the biological effect of TiO2 nanoparticles in neural cells. We used neuroblastoma SH-SY5Y cells treated with 25 nm TiO2 nanoparticles (TiO2 P25; 1-100 µg.mL(-1); 24-72 h). We measured dehydrogenase activity, glutathione metabolism, DNA damage and mitochondrial respiration. After treatment of SH-SY5Y cells with TiO2 P25, we found a dose- and time-dependent decrease in dehydrogenase activity together with nuclear changes. Interestingly, 100 µg.mL(-1) TiO2 P25 caused a significant increase of glutathione levels (p &lt; 0.001) linked with increased glutamate-cysteine ligase expression after both 24 and 48 h. Furthermore, as the first study at all, we observed the significant decreases of mitochondrial respiration in SH-SY5Y cells caused by treatments with 10 and 100 µg.mL(-1) TiO2 P25 after 48 and 72 h. In conclusion, our study brings new finding of occurrence of decreased mitochondrial respiration without glutathione depletion in SH-SY5Y cells contributing to the understanding of TiO2 P25 toxicity in neural cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40500 - Other agricultural sciences

Projekt

<a href="/cs/project/EF17_048%2F0007421" target="_blank" >EF17_048/0007421: Posilování mezioborové spolupráce ve výzkumu nanomateriálů a při studiu jejich účinků na živé organismy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food and Chemical Toxicology

ISSN

0278-6915

e-ISSN

1873-6351

Svazek periodika

202

Číslo periodika v rámci svazku

August 2025

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

115496

Kód UT WoS článku

001488077800001

EID výsledku v databázi Scopus

2-s2.0-105003810333