Denitrification assays for testing effects of xenobiotics on aquatic denitrification and their degradation in aquatic environments

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26110%2F23%3APU148665" target="_blank" >RIV/00216305:26110/23:PU148665 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/23:00132248

Výsledek na webu

<a href="https://www.mdpi.com/2073-4441/15/14/2536" target="_blank" >https://www.mdpi.com/2073-4441/15/14/2536</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/w15142536" target="_blank" >10.3390/w15142536</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Denitrification assays for testing effects of xenobiotics on aquatic denitrification and their degradation in aquatic environments

Popis výsledku v původním jazyce

We developed, tested, and optimized two laboratory denitrification assays for both managers and scientists to assess the effects of xenobiotics on the denitrification process over 7 days (short batch assay, SBA) and 28 days (long semi-continuous assay, LSA). The assays facilitate (1) measuring the efficiency of nitrate removal under the influence of xenobiotics, (2) determining the removal of the tested xenobiotics via adsorption or biotic decomposition, and (3) testing the influencing parameters for optimizing the denitrification process. The adsorption of the xenobiotics was assessed by inhibiting all biological processes through the addition of HgCl2. Our tests demonstrate that the ratio of the initial nitrate concentration to the amount of bioavailable organic matter provided is essential to avoid organic carbon or nitrate limitation. While a pH less than 7 resulted in decreased denitrification, a pH more than 8 let to nitrite accumulation, indicating incomplete denitrification. Over durations of more than a week, weekly replenishments of the nitrate and HgCl2 and weekly purging with argon gas to reduce the oxygen concentrations are needed. The assays provide information about the accumulation of xenobiotics in the bioreactors that is necessary for the environmentally friendly treatment of the bioreactor fillings and provide insight into the potential of the bioreactors to remove pesticides from polluted water resources.

Název v anglickém jazyce

Denitrification assays for testing effects of xenobiotics on aquatic denitrification and their degradation in aquatic environments

Popis výsledku anglicky

We developed, tested, and optimized two laboratory denitrification assays for both managers and scientists to assess the effects of xenobiotics on the denitrification process over 7 days (short batch assay, SBA) and 28 days (long semi-continuous assay, LSA). The assays facilitate (1) measuring the efficiency of nitrate removal under the influence of xenobiotics, (2) determining the removal of the tested xenobiotics via adsorption or biotic decomposition, and (3) testing the influencing parameters for optimizing the denitrification process. The adsorption of the xenobiotics was assessed by inhibiting all biological processes through the addition of HgCl2. Our tests demonstrate that the ratio of the initial nitrate concentration to the amount of bioavailable organic matter provided is essential to avoid organic carbon or nitrate limitation. While a pH less than 7 resulted in decreased denitrification, a pH more than 8 let to nitrite accumulation, indicating incomplete denitrification. Over durations of more than a week, weekly replenishments of the nitrate and HgCl2 and weekly purging with argon gas to reduce the oxygen concentrations are needed. The assays provide information about the accumulation of xenobiotics in the bioreactors that is necessary for the environmentally friendly treatment of the bioreactor fillings and provide insight into the potential of the bioreactors to remove pesticides from polluted water resources.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10503 - Water resources

Projekt

<a href="/cs/project/EF17_043%2F0009632" target="_blank" >EF17_043/0009632: CETOCOEN Excellence</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Water

ISSN

2073-4441

e-ISSN

—

Svazek periodika

15

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

„“-„“

Kód UT WoS článku

001073253600001

EID výsledku v databázi Scopus

2-s2.0-85166200869