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Advances in Digital Holographic Microscopy: Coherence-Controlled Microscope

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F11%3APU95917" target="_blank" >RIV/00216305:26210/11:PU95917 - isvavai.cz</a>

  • Výsledek na webu

    <a href="http://spie.org/x648.html?product_id=888733" target="_blank" >http://spie.org/x648.html?product_id=888733</a>

  • DOI - Digital Object Identifier

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Advances in Digital Holographic Microscopy: Coherence-Controlled Microscope

  • Popis výsledku v původním jazyce

    Digital holographic microscope (DHM) allows for direct accessing to the quantitative phase contrast. In this way it becomes a completely non-invasive tool for a contrast living cells observation and the dry-mass density distribution of a cell measurement. Current DHMs use the off-axis holographic setup based on conventional interferometers, which properly work with the coherent illumination. However, this type of illumination is inconvenient for microscopy because of the coherence noise, unwanted interference and a worse lateral resolution. An uncompromising solution to this problem is a DHM based on the Leith achromatic interferometer. While in this setup an off-axis hologram is formed with an arbitrary degree of the illumination coherence, the resolution and the image quality typical for an incoherent-light widefield microscopy can be achieved. Moreover, coherence gating can be introduced which makes the DHM image similar to that of confocal microscope. Hence the character of the DHM image is controlled by the coherence between two extremes: fully coherent-light holography and confocal-microscope-like imaging. The above described possibilities were proved experimentally by the coherence-controlled holographic microscope, which is a DHM based on the achromaticinterferometer. Living cancer cells were observed and their motility was evaluated in the quantitative phase contrast. The presence of the coherence gate was demonstrated by imaging of model samples through a scattering layer.

  • Název v anglickém jazyce

    Advances in Digital Holographic Microscopy: Coherence-Controlled Microscope

  • Popis výsledku anglicky

    Digital holographic microscope (DHM) allows for direct accessing to the quantitative phase contrast. In this way it becomes a completely non-invasive tool for a contrast living cells observation and the dry-mass density distribution of a cell measurement. Current DHMs use the off-axis holographic setup based on conventional interferometers, which properly work with the coherent illumination. However, this type of illumination is inconvenient for microscopy because of the coherence noise, unwanted interference and a worse lateral resolution. An uncompromising solution to this problem is a DHM based on the Leith achromatic interferometer. While in this setup an off-axis hologram is formed with an arbitrary degree of the illumination coherence, the resolution and the image quality typical for an incoherent-light widefield microscopy can be achieved. Moreover, coherence gating can be introduced which makes the DHM image similar to that of confocal microscope. Hence the character of the DHM image is controlled by the coherence between two extremes: fully coherent-light holography and confocal-microscope-like imaging. The above described possibilities were proved experimentally by the coherence-controlled holographic microscope, which is a DHM based on the achromaticinterferometer. Living cancer cells were observed and their motility was evaluated in the quantitative phase contrast. The presence of the coherence gate was demonstrated by imaging of model samples through a scattering layer.

Klasifikace

  • Druh

    D - Stať ve sborníku

  • CEP obor

    BH - Optika, masery a lasery

  • OECD FORD obor

Návaznosti výsledku

  • Projekt

  • Návaznosti

    Z - Vyzkumny zamer (s odkazem do CEZ)

Ostatní

  • Rok uplatnění

    2011

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název statě ve sborníku

    Proceedings of SPIE

  • ISBN

    9780819486646

  • ISSN

    0277-786X

  • e-ISSN

  • Počet stran výsledku

    328

  • Strana od-do

    "807402-1"-"807402-7"

  • Název nakladatele

    SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA

  • Místo vydání

    BELLINGHAM, USA

  • Místo konání akce

    Praha

  • Datum konání akce

    18. 4. 2011

  • Typ akce podle státní příslušnosti

    WRD - Celosvětová akce

  • Kód UT WoS článku

    000293696400001