Advances in Digital Holographic Microscopy: Coherence-Controlled Microscope
Identifikátory výsledku
Kód výsledku v IS VaVaI
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Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Advances in Digital Holographic Microscopy: Coherence-Controlled Microscope
Popis výsledku v původním jazyce
Digital holographic microscope (DHM) allows for direct accessing to the quantitative phase contrast. In this way it becomes a completely non-invasive tool for a contrast living cells observation and the dry-mass density distribution of a cell measurement. Current DHMs use the off-axis holographic setup based on conventional interferometers, which properly work with the coherent illumination. However, this type of illumination is inconvenient for microscopy because of the coherence noise, unwanted interference and a worse lateral resolution. An uncompromising solution to this problem is a DHM based on the Leith achromatic interferometer. While in this setup an off-axis hologram is formed with an arbitrary degree of the illumination coherence, the resolution and the image quality typical for an incoherent-light widefield microscopy can be achieved. Moreover, coherence gating can be introduced which makes the DHM image similar to that of confocal microscope. Hence the character of the DHM image is controlled by the coherence between two extremes: fully coherent-light holography and confocal-microscope-like imaging. The above described possibilities were proved experimentally by the coherence-controlled holographic microscope, which is a DHM based on the achromaticinterferometer. Living cancer cells were observed and their motility was evaluated in the quantitative phase contrast. The presence of the coherence gate was demonstrated by imaging of model samples through a scattering layer.
Název v anglickém jazyce
Advances in Digital Holographic Microscopy: Coherence-Controlled Microscope
Popis výsledku anglicky
Digital holographic microscope (DHM) allows for direct accessing to the quantitative phase contrast. In this way it becomes a completely non-invasive tool for a contrast living cells observation and the dry-mass density distribution of a cell measurement. Current DHMs use the off-axis holographic setup based on conventional interferometers, which properly work with the coherent illumination. However, this type of illumination is inconvenient for microscopy because of the coherence noise, unwanted interference and a worse lateral resolution. An uncompromising solution to this problem is a DHM based on the Leith achromatic interferometer. While in this setup an off-axis hologram is formed with an arbitrary degree of the illumination coherence, the resolution and the image quality typical for an incoherent-light widefield microscopy can be achieved. Moreover, coherence gating can be introduced which makes the DHM image similar to that of confocal microscope. Hence the character of the DHM image is controlled by the coherence between two extremes: fully coherent-light holography and confocal-microscope-like imaging. The above described possibilities were proved experimentally by the coherence-controlled holographic microscope, which is a DHM based on the achromaticinterferometer. Living cancer cells were observed and their motility was evaluated in the quantitative phase contrast. The presence of the coherence gate was demonstrated by imaging of model samples through a scattering layer.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
BH - Optika, masery a lasery
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
Proceedings of SPIE
ISBN
9780819486646
ISSN
0277-786X
e-ISSN
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Počet stran výsledku
328
Strana od-do
"807402-1"-"807402-7"
Název nakladatele
SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
Místo vydání
BELLINGHAM, USA
Místo konání akce
Praha
Datum konání akce
18. 4. 2011
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
000293696400001