Surface Passivation Improves the Synthesis of Highly Stable and Specific DNA-Functionalized Gold Nanoparticles with Variable DNA Density

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F15%3APU114038" target="_blank" >RIV/00216305:26210/15:PU114038 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1021/acsami.5b01191" target="_blank" >http://dx.doi.org/10.1021/acsami.5b01191</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsami.5b01191" target="_blank" >10.1021/acsami.5b01191</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Surface Passivation Improves the Synthesis of Highly Stable and Specific DNA-Functionalized Gold Nanoparticles with Variable DNA Density

Popis výsledku v původním jazyce

We report a novel and multifaceted approach for the quick synthesis of highly stable single-stranded DNA (ssDNA) functionalized gold nanoparticles (AuNPs). The method is based on the combined effect of surface passivation by (1-mercaptoundec-11-yl)hexa(ethylene glycol) and low pH conditions, does not require any salt pretreatment or high excess of ssDNA, and can be generalized for oligonucleotides of any length or base sequence. The synthesized ssDNA-coated AuNPs conjugates are stable at salt concentrations as high as 3.0 M, and also functional and specific toward DNA–DNA hybridization, as shown from UV–vis spectrophotometry, scanning electron microscopy, gel electrophoresis, fluorescence, and small angle X-ray scattering based analyses. The method is highly flexible and shows an additional advantage of creating ssDNA-AuNP conjugates with a predefined number of ssDNA strands per particle. Its simplicity and tenability make it widely applicable to diverse biosensing applications involving ssDNA functionalized AuNPs.

Název v anglickém jazyce

Surface Passivation Improves the Synthesis of Highly Stable and Specific DNA-Functionalized Gold Nanoparticles with Variable DNA Density

Popis výsledku anglicky

We report a novel and multifaceted approach for the quick synthesis of highly stable single-stranded DNA (ssDNA) functionalized gold nanoparticles (AuNPs). The method is based on the combined effect of surface passivation by (1-mercaptoundec-11-yl)hexa(ethylene glycol) and low pH conditions, does not require any salt pretreatment or high excess of ssDNA, and can be generalized for oligonucleotides of any length or base sequence. The synthesized ssDNA-coated AuNPs conjugates are stable at salt concentrations as high as 3.0 M, and also functional and specific toward DNA–DNA hybridization, as shown from UV–vis spectrophotometry, scanning electron microscopy, gel electrophoresis, fluorescence, and small angle X-ray scattering based analyses. The method is highly flexible and shows an additional advantage of creating ssDNA-AuNP conjugates with a predefined number of ssDNA strands per particle. Its simplicity and tenability make it widely applicable to diverse biosensing applications involving ssDNA functionalized AuNPs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10302 - Condensed matter physics (including formerly solid state physics, supercond.)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS applied materials & interfaces

ISSN

1944-8244

e-ISSN

1944-8252

Svazek periodika

7

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

7033-7040

Kód UT WoS článku

000352246700080

EID výsledku v databázi Scopus

2-s2.0-84926364843