Characteristics of synovial fluid required for optimization of lubrication fluid for biotribological experiments

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F16%3APU119595" target="_blank" >RIV/00216305:26210/16:PU119595 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/17:73587037

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33663/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33663/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jbm.b.33663" target="_blank" >10.1002/jbm.b.33663</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characteristics of synovial fluid required for optimization of lubrication fluid for biotribological experiments

Popis výsledku v původním jazyce

Wear testing of total joint replacement (TJR) is mandatory in preclinical testing before implantation of TJR into the human body. Testing is governed by current international standards that recommend bovine serum (BS) as a lubricating fluid to replace synovial fluid (SF). Recently, the use of BS has been criticized because of differences in content, fluid characteristics, and nonhuman origin. As a result, a more realistic lubricant mimicking SF is needed. To define SF composition, we analyzed SF obtained during revisions of total hip and knee arthroplasties and compared it with SF obtained during primary arthroplasties and from patients without TJR. Samples were acquired from 152 patients. We found that the median total protein concentration for all SF was 36.8 mg/mL, which is significantly higher than concentrations currently recommended by the ISO standards. The gama globulin concentration was significantly higher and the phospholipid concentration significantly lower in patients with revision of TJR compared with patients without TJR. No significant difference was found in hyaluronic acid concentration and viscosity among the groups. Our results support the need to improve the definition of a more clinically relevant wear testing lubricant in the ISO standards.

Název v anglickém jazyce

Characteristics of synovial fluid required for optimization of lubrication fluid for biotribological experiments

Popis výsledku anglicky

Wear testing of total joint replacement (TJR) is mandatory in preclinical testing before implantation of TJR into the human body. Testing is governed by current international standards that recommend bovine serum (BS) as a lubricating fluid to replace synovial fluid (SF). Recently, the use of BS has been criticized because of differences in content, fluid characteristics, and nonhuman origin. As a result, a more realistic lubricant mimicking SF is needed. To define SF composition, we analyzed SF obtained during revisions of total hip and knee arthroplasties and compared it with SF obtained during primary arthroplasties and from patients without TJR. Samples were acquired from 152 patients. We found that the median total protein concentration for all SF was 36.8 mg/mL, which is significantly higher than concentrations currently recommended by the ISO standards. The gama globulin concentration was significantly higher and the phospholipid concentration significantly lower in patients with revision of TJR compared with patients without TJR. No significant difference was found in hyaluronic acid concentration and viscosity among the groups. Our results support the need to improve the definition of a more clinically relevant wear testing lubricant in the ISO standards.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30211 - Orthopaedics

Projekt

<a href="/cs/project/NT14267" target="_blank" >NT14267: Vliv složení kloubní kapaliny na utváření mazacího filmu v TEP kyčle</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of biomedical materials research. Part B, Applied biomaterials (Electronic)

ISSN

1552-4973

e-ISSN

1552-4981

Svazek periodika

105

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1422-1431

Kód UT WoS článku

000407055400011

EID výsledku v databázi Scopus

2-s2.0-84963603091