Modelling invasive pathogen load from non-destructive sampling data

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F19%3APU131273" target="_blank" >RIV/00216305:26210/19:PU131273 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081766:_____/19:00499378 RIV/62157124:16270/19:43877448 RIV/00216224:14110/19:00113549

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0022519318306258?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0022519318306258?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jtbi.2018.12.026" target="_blank" >10.1016/j.jtbi.2018.12.026</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Modelling invasive pathogen load from non-destructive sampling data

Popis výsledku v původním jazyce

Where microbes colonizing skin surface may help maintain organism homeostasis, those that invade living skin layers cause disease. In bats, white-nose syndrome is a fungal skin infection that affects animals during hibernation and may lead to mortality in severe cases. Here, we inferred the amount of fungus that had invaded skin tissue of diseased animals. We used simulations to estimate the unobserved disease severity in a non-lethal wing punch biopsy and to relate the simulated pathology to the measured fungal load in paired biopsies. We found that a single white-nose syndrome skin lesion packed with spores and hyphae of the causative agent, Pseudogymnoascus destructans, contains 48.93 pg of the pathogen DNA, which amounts to about 1560 P destructans genomes in one skin lesion. Relating the information to the known UV fluorescence in Nearctic and Palearctic bats shows that Nearctic bats carry about 1.7 mu g of fungal DNA per cm(2), whereas Palearctic bats have 0.04 g cm(-2) of P. destructans DNA. With the information on the fungal load that had invaded the host skin, the researchers can now calculate disease severity as a function of invasive fungal growth using non-destructive UV light transillumination of each bat's wing membranes. Our results will enable and promote thorough disease severity assessment in protected bat species without the need for extensive animal and laboratory labor sacrifices.

Název v anglickém jazyce

Modelling invasive pathogen load from non-destructive sampling data

Popis výsledku anglicky

Where microbes colonizing skin surface may help maintain organism homeostasis, those that invade living skin layers cause disease. In bats, white-nose syndrome is a fungal skin infection that affects animals during hibernation and may lead to mortality in severe cases. Here, we inferred the amount of fungus that had invaded skin tissue of diseased animals. We used simulations to estimate the unobserved disease severity in a non-lethal wing punch biopsy and to relate the simulated pathology to the measured fungal load in paired biopsies. We found that a single white-nose syndrome skin lesion packed with spores and hyphae of the causative agent, Pseudogymnoascus destructans, contains 48.93 pg of the pathogen DNA, which amounts to about 1560 P destructans genomes in one skin lesion. Relating the information to the known UV fluorescence in Nearctic and Palearctic bats shows that Nearctic bats carry about 1.7 mu g of fungal DNA per cm(2), whereas Palearctic bats have 0.04 g cm(-2) of P. destructans DNA. With the information on the fungal load that had invaded the host skin, the researchers can now calculate disease severity as a function of invasive fungal growth using non-destructive UV light transillumination of each bat's wing membranes. Our results will enable and promote thorough disease severity assessment in protected bat species without the need for extensive animal and laboratory labor sacrifices.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10102 - Applied mathematics

Projekt

<a href="/cs/project/GA17-20286S" target="_blank" >GA17-20286S: Fyziologie hibernace netopýrů s ohledem na dopad mnohočetných stresorů</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Theoretical Biology

ISSN

0022-5193

e-ISSN

1095-8541

Svazek periodika

neuveden

Číslo periodika v rámci svazku

464

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

98-103

Kód UT WoS článku

000457204500009

EID výsledku v databázi Scopus

2-s2.0-85059111717