The Effect of Albumin and γ-globulin on Synovial Fluid Lubrication: Implication for Knee Joint Replacements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F21%3APU137355" target="_blank" >RIV/00216305:26210/21:PU137355 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/21:73608615

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1751616120306652" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1751616120306652</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jmbbm.2020.104117" target="_blank" >10.1016/j.jmbbm.2020.104117</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Effect of Albumin and γ-globulin on Synovial Fluid Lubrication: Implication for Knee Joint Replacements

Popis výsledku v původním jazyce

Total knee arthroplasty has become a routine procedure for patients suffering from joint diseases. Although the number of operations continuously increases, a limited service-life of implants represents a persisting challenge for scientists. Understanding of lubrication may help to suitably explain tribological processes on the way to replacements that become durable well into the third decade of service. The aim of the present study is to assess the formation of protein lubricating film in the knee implant. A developed knee simulator was used to observe the contact of real femoral and transparent polymer tibial component using fluorescent microscopy. The contact was lubricated by various protein solutions with attention to the behaviour of albumin and γ-globulin. In order to suitably mimic a human synovial fluid, hyaluronic acid and phospholipids were subsequently added to the solutions. Further, the change in shape and the migration of the contact zone were studied. The results showed considerable appearance differences of the contact over the swing phase of the simplified gait cycle. Regarding film formation, a strong interaction of the various molecules of synovial fluid was observed. It was found that the thickness of the lubricating layer stabilizes within around 50 seconds. Throughout the contact zone, protein agglomerations were present and could be clearly visualised using the applied optical technique.

Název v anglickém jazyce

The Effect of Albumin and γ-globulin on Synovial Fluid Lubrication: Implication for Knee Joint Replacements

Popis výsledku anglicky

Total knee arthroplasty has become a routine procedure for patients suffering from joint diseases. Although the number of operations continuously increases, a limited service-life of implants represents a persisting challenge for scientists. Understanding of lubrication may help to suitably explain tribological processes on the way to replacements that become durable well into the third decade of service. The aim of the present study is to assess the formation of protein lubricating film in the knee implant. A developed knee simulator was used to observe the contact of real femoral and transparent polymer tibial component using fluorescent microscopy. The contact was lubricated by various protein solutions with attention to the behaviour of albumin and γ-globulin. In order to suitably mimic a human synovial fluid, hyaluronic acid and phospholipids were subsequently added to the solutions. Further, the change in shape and the migration of the contact zone were studied. The results showed considerable appearance differences of the contact over the swing phase of the simplified gait cycle. Regarding film formation, a strong interaction of the various molecules of synovial fluid was observed. It was found that the thickness of the lubricating layer stabilizes within around 50 seconds. Throughout the contact zone, protein agglomerations were present and could be clearly visualised using the applied optical technique.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20301 - Mechanical engineering

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of the mechanical behavior of biomedical materials

ISSN

1751-6161

e-ISSN

1878-0180

Svazek periodika

113

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

104117-104117

Kód UT WoS článku

000599818300004

EID výsledku v databázi Scopus

2-s2.0-85092361148