Bioprospecting of biosurfactant-producing bacteria for hydrocarbon bioremediation: Optimization and characterization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F23%3APU150495" target="_blank" >RIV/00216305:26210/23:PU150495 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11814-023-1418-y" target="_blank" >https://link.springer.com/article/10.1007/s11814-023-1418-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11814-023-1418-y" target="_blank" >10.1007/s11814-023-1418-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bioprospecting of biosurfactant-producing bacteria for hydrocarbon bioremediation: Optimization and characterization

Popis výsledku v původním jazyce

Biosurfactants have been found capable of replacing synthetic surfactants which include ongoing bioprospecting of biosurfactant-producing bacteria as well as process optimization for maximum biosurfactant production. In this study, five morphologically distinct actinomycete strains isolated from hydrocarbon-polluted soil collected from an oil spill surface in Southeastern Algeria were tested for their ability to produce biosurfactants using preliminary biosurfactant screening assays. The 7SDS strain was selected as the most promising biosurfactant producer due to its greatest oil displacement diameter (7.83 & PLUSMN;0.15 cm), emulsification index (59.66 & PLUSMN;0.44%), and enhanced surface tension reduction (30.04 & PLUSMN;0.51 mN/m); it was identified as Streptomyces thinghirensis 7SDS using 16S rDNA sequence analysis. The 7SDS strain's biosurfactant production was optimized using the Face-centered central composite design (CCD) based on response surface methodology (RSM). To this end, five independent factors, i.e., residual frying oil, used engine oil, whey, CS filtrate, and incubation time, were assessed. The RSM's model predicted a surface tension of 27.48 mN/m using 2.44% (v/v) residual frying oil, 0.35% (v/v) used motor oil, 0.83% (v/v) whey, 0.39% (v/v) CS filtrate, and an incubation time of 219.3 h. The optimized medium produced 8.79 g/L of biosurfactant. The produced biosurfactant allows one to reduce the surface tension of distilled water from 70.86 mN/m to 27.96 mN/m at a critical micelle concentration of 350 mg/L, even over a wide range of pH (2.0-12.0), temperature (4-120 & DEG;C), and salinity (2-12%, W/V). Biochemical (Biuret, phenol-sulfuric acid, and phosphate tests) and compositional (FTIR and GC-MS) characterizations confirmed the phospholipid nature of the produced biosurfactant. Interestingly, the produced BS demonstrated significant antimicrobial activity as well as intriguing activity in removing hydrocarbons from polluted soil. Because of t

Název v anglickém jazyce

Bioprospecting of biosurfactant-producing bacteria for hydrocarbon bioremediation: Optimization and characterization

Popis výsledku anglicky

Biosurfactants have been found capable of replacing synthetic surfactants which include ongoing bioprospecting of biosurfactant-producing bacteria as well as process optimization for maximum biosurfactant production. In this study, five morphologically distinct actinomycete strains isolated from hydrocarbon-polluted soil collected from an oil spill surface in Southeastern Algeria were tested for their ability to produce biosurfactants using preliminary biosurfactant screening assays. The 7SDS strain was selected as the most promising biosurfactant producer due to its greatest oil displacement diameter (7.83 & PLUSMN;0.15 cm), emulsification index (59.66 & PLUSMN;0.44%), and enhanced surface tension reduction (30.04 & PLUSMN;0.51 mN/m); it was identified as Streptomyces thinghirensis 7SDS using 16S rDNA sequence analysis. The 7SDS strain's biosurfactant production was optimized using the Face-centered central composite design (CCD) based on response surface methodology (RSM). To this end, five independent factors, i.e., residual frying oil, used engine oil, whey, CS filtrate, and incubation time, were assessed. The RSM's model predicted a surface tension of 27.48 mN/m using 2.44% (v/v) residual frying oil, 0.35% (v/v) used motor oil, 0.83% (v/v) whey, 0.39% (v/v) CS filtrate, and an incubation time of 219.3 h. The optimized medium produced 8.79 g/L of biosurfactant. The produced biosurfactant allows one to reduce the surface tension of distilled water from 70.86 mN/m to 27.96 mN/m at a critical micelle concentration of 350 mg/L, even over a wide range of pH (2.0-12.0), temperature (4-120 & DEG;C), and salinity (2-12%, W/V). Biochemical (Biuret, phenol-sulfuric acid, and phosphate tests) and compositional (FTIR and GC-MS) characterizations confirmed the phospholipid nature of the produced biosurfactant. Interestingly, the produced BS demonstrated significant antimicrobial activity as well as intriguing activity in removing hydrocarbons from polluted soil. Because of t

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20400 - Chemical engineering

Projekt

<a href="/cs/project/EF15_003%2F0000456" target="_blank" >EF15_003/0000456: Laboratoř integrace procesů pro trvalou udržitelnost</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

KOREAN JOURNAL OF CHEMICAL ENGINEERING

ISSN

0256-1115

e-ISSN

1975-7220

Svazek periodika

10

Číslo periodika v rámci svazku

40

Stát vydavatele periodika

KR - Korejská republika

Počet stran výsledku

16

Strana od-do

2497-2512

Kód UT WoS článku

001013520700010

EID výsledku v databázi Scopus

2-s2.0-85162040587