Parallel-Mode Confocal Microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F99%3APU20998" target="_blank" >RIV/00216305:26210/99:PU20998 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Parallel-Mode Confocal Microscopy

Popis výsledku v původním jazyce

Conventional confocal microscopes are based on the dual scanning of a specimen by the images of a point source and of a point detector. Hence, their imaging mode is serial, i.e., the confocal image is obtained from the sequence of single-point or multiple-point partial images. Parallel-mode confocal imaging is possible on the basis of broad-source image-plane holography. We adapted this classical-holography technique for real-time reflected-light microscopy. The imaging speed of the parallel-mode conffocal microscope is not limited by any part of the optical system, but only by the image detection and storage systems. Both the image phase and the image amplitude are reconstructed from the interference signal. It was verified both theoretically and experimentally that the main imaging parameters of the microscope are comparable with those of a conventional confocal microscope. The only exception is the imaging speed, which can be much higher.

Název v anglickém jazyce

Parallel-Mode Confocal Microscopy

Popis výsledku anglicky

Conventional confocal microscopes are based on the dual scanning of a specimen by the images of a point source and of a point detector. Hence, their imaging mode is serial, i.e., the confocal image is obtained from the sequence of single-point or multiple-point partial images. Parallel-mode confocal imaging is possible on the basis of broad-source image-plane holography. We adapted this classical-holography technique for real-time reflected-light microscopy. The imaging speed of the parallel-mode conffocal microscope is not limited by any part of the optical system, but only by the image detection and storage systems. Both the image phase and the image amplitude are reconstructed from the interference signal. It was verified both theoretically and experimentally that the main imaging parameters of the microscope are comparable with those of a conventional confocal microscope. The only exception is the imaging speed, which can be much higher.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BH - Optika, masery a lasery

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

1999

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Optical Engineering

ISSN

0091-3286

e-ISSN

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Svazek periodika

38

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

1635-1639

Kód UT WoS článku

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EID výsledku v databázi Scopus

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