Srovnání biosenzorových a chromatografických metod pro detekci pesticidů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F05%3APU51991" target="_blank" >RIV/00216305:26220/05:PU51991 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/26234386:_____/05:#0000010

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of biosensoric and chromatographic methods for the detection of pesticides

Popis výsledku v původním jazyce

Screen-printed biosensors with immobilized acetylcholine esterase (AChE) were used for measuring fruit and vegetable samples that had first been analysed using gas and highperformance liquid chromatography. The output signal for the biosensors is the current, which is used to calculate relative inhibition (RI), a measured quantity. RI is proportional to the inhibiting (toxic) effect of organophosphates and carbamates. Measurements with AChE biosensors are not easily reproducible. This problem is soolvedby the choice of an arbitrary toxicity standard of 1.25 uM Syntostigmin. Measurements were evaluated by the ratio of the relative inhibition of the sample against the relative inhibition of Syntostigmin. Results obtained from the biosensor matchthose ofchromatography in 19 out of 38 total measurements made and for nine out of 19 positive samples. The confirmation rate was 50%. Future work must check the limit of 0.1 and the independent control of inhibiting pesticides contents after me

Název v anglickém jazyce

Comparison of biosensoric and chromatographic methods for the detection of pesticides

Popis výsledku anglicky

Screen-printed biosensors with immobilized acetylcholine esterase (AChE) were used for measuring fruit and vegetable samples that had first been analysed using gas and highperformance liquid chromatography. The output signal for the biosensors is the current, which is used to calculate relative inhibition (RI), a measured quantity. RI is proportional to the inhibiting (toxic) effect of organophosphates and carbamates. Measurements with AChE biosensors are not easily reproducible. This problem is soolvedby the choice of an arbitrary toxicity standard of 1.25 uM Syntostigmin. Measurements were evaluated by the ratio of the relative inhibition of the sample against the relative inhibition of Syntostigmin. Results obtained from the biosensor matchthose ofchromatography in 19 out of 38 total measurements made and for nine out of 19 positive samples. The confirmation rate was 50%. Future work must check the limit of 0.1 and the independent control of inhibiting pesticides contents after me

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JB - Senzory, čidla, měření a regulace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Environmental Analytical Chemistry

ISSN

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e-ISSN

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Svazek periodika

85

Číslo periodika v rámci svazku

12-13

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

885-892

Kód UT WoS článku

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EID výsledku v databázi Scopus

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