The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F15%3APU116286" target="_blank" >RIV/00216305:26220/15:PU116286 - isvavai.cz</a>

Výsledek na webu

<a href="https://ieeexplore.ieee.org/document/7411094" target="_blank" >https://ieeexplore.ieee.org/document/7411094</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1109/CIC.2015.7411094" target="_blank" >10.1109/CIC.2015.7411094</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart

Popis výsledku v původním jazyce

Optical mapping of heart electrical activity in Langendorff-perfused isolated hearts is based on voltage-sensitive dyes, of which the most commonly used one is di-4-ANEPPS. Prolongation of action potential duration by di-4-ANEPPS was reported in isolated cells; however, this phenomenon was not observed in the whole heart model. In order to search for other adverse phenomena of di-4-ANEPPS use, its effect on heart rate variability (HRV) was investigated during staining and washout periods in five New Zealand White rabbit isolated hearts. Time domain, frequency domain and non-linear HRV parameters (21 in total) revealed that there are no significant changes between control, staining and washout periods. Voltage-sensitive dye di-4-ANEPPS does not affect HRV when applied into the isolated heart coronary system. It can be concluded that di-4-ANEPPS may be safely used in studies combining optical mapping and HRV analysis.

Název v anglickém jazyce

The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart

Popis výsledku anglicky

Optical mapping of heart electrical activity in Langendorff-perfused isolated hearts is based on voltage-sensitive dyes, of which the most commonly used one is di-4-ANEPPS. Prolongation of action potential duration by di-4-ANEPPS was reported in isolated cells; however, this phenomenon was not observed in the whole heart model. In order to search for other adverse phenomena of di-4-ANEPPS use, its effect on heart rate variability (HRV) was investigated during staining and washout periods in five New Zealand White rabbit isolated hearts. Time domain, frequency domain and non-linear HRV parameters (21 in total) revealed that there are no significant changes between control, staining and washout periods. Voltage-sensitive dye di-4-ANEPPS does not affect HRV when applied into the isolated heart coronary system. It can be concluded that di-4-ANEPPS may be safely used in studies combining optical mapping and HRV analysis.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

<a href="/cs/project/GAP102%2F12%2F2034" target="_blank" >GAP102/12/2034: Analýza vztahu mezi elektrickými ději a průtokem krve u srdečních komor</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Computing in Cardiology 2015

ISBN

978-1-4799-4347-0

ISSN

—

e-ISSN

—

Počet stran výsledku

4

Strana od-do

1049-1052

Název nakladatele

Neuveden

Místo vydání

Nice, Francie

Místo konání akce

Nice, France

Datum konání akce

6. 9. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000380374700264