Carbon dots for in vivo fluorescence imaging of adipose tissue-derived mesenchymal stromal cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F19%3APU134870" target="_blank" >RIV/00216305:26220/19:PU134870 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/19:10400453 RIV/61989592:15110/19:73595150 RIV/61989592:15310/19:73595150

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S0008622319305342" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0008622319305342</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.carbon.2019.05.061" target="_blank" >10.1016/j.carbon.2019.05.061</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Carbon dots for in vivo fluorescence imaging of adipose tissue-derived mesenchymal stromal cells

Popis výsledku v původním jazyce

Tissue regeneration based on stem cell therapy is one of the most rapidly developing fields of modern medicine. Several properties of human mesenchymal stromal cells (MSCs), such as tropism toward a tumor or injury site, make them promising candidates for regenerative medicine, targeted therapy, or treating injured tissues. However, to fully understand the role of stem cells in therapeutic function, their visualization in vivo is essential. Here, we describe, for the first time, the use of biocompatible quaternized carbon dots (QCDs) as a novel stem-cell tracking probe for in vivo fluorescence imaging of transplanted human MSCs. By studying the in vitro cytotoxicity, intracellular distribution, and precise uptake mechanism, we showed that QCDs had a high biocompatibility and excellent fluorescence properties after 24 h incubation with MSCs. Further to demonstrate the in vivo feasibility of the system, QCD-labeled MSCs (100 μg/mL of QCDs, 24 h incubation time) were transplanted subcutaneously into an immunodeficient mouse and visualized by optical in vivo imaging. The labeled cells were strongly fluorescent, allowing their semi-quantitative detection. Moreover, the homing of intravenously transplanted QCD-labeled MSCs into the solid tumor was clearly shown. The results demonstrated that QCD-labeling of human MSCs is a highly promising approach for in vivo tracking during stem cell therapy.

Název v anglickém jazyce

Carbon dots for in vivo fluorescence imaging of adipose tissue-derived mesenchymal stromal cells

Popis výsledku anglicky

Tissue regeneration based on stem cell therapy is one of the most rapidly developing fields of modern medicine. Several properties of human mesenchymal stromal cells (MSCs), such as tropism toward a tumor or injury site, make them promising candidates for regenerative medicine, targeted therapy, or treating injured tissues. However, to fully understand the role of stem cells in therapeutic function, their visualization in vivo is essential. Here, we describe, for the first time, the use of biocompatible quaternized carbon dots (QCDs) as a novel stem-cell tracking probe for in vivo fluorescence imaging of transplanted human MSCs. By studying the in vitro cytotoxicity, intracellular distribution, and precise uptake mechanism, we showed that QCDs had a high biocompatibility and excellent fluorescence properties after 24 h incubation with MSCs. Further to demonstrate the in vivo feasibility of the system, QCD-labeled MSCs (100 μg/mL of QCDs, 24 h incubation time) were transplanted subcutaneously into an immunodeficient mouse and visualized by optical in vivo imaging. The labeled cells were strongly fluorescent, allowing their semi-quantitative detection. Moreover, the homing of intravenously transplanted QCD-labeled MSCs into the solid tumor was clearly shown. The results demonstrated that QCD-labeling of human MSCs is a highly promising approach for in vivo tracking during stem cell therapy.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10610 - Biophysics

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

CARBON

ISSN

0008-6223

e-ISSN

1873-3891

Svazek periodika

152

Číslo periodika v rámci svazku

2019

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

434-443

Kód UT WoS článku

000483384900047

EID výsledku v databázi Scopus

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