Deeper below the surface – transcriptional changes of selected Clostridium beijerinckii NRRL B-598 genes induced by butanol shock

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F20%3APU137954" target="_blank" >RIV/00216305:26220/20:PU137954 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1002/mbo3.1146" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/mbo3.1146</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/mbo3.1146" target="_blank" >10.1002/mbo3.1146</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Deeper below the surface – transcriptional changes of selected Clostridium beijerinckii NRRL B-598 genes induced by butanol shock

Popis výsledku v původním jazyce

The main bottleneck in the return of industrial butanol production from renewable feedstock, by acetone-butanol-ethanol (ABE) fermentation by clostridia, such as Clostridium beijerinckii, is the low final butanol concentration. The problem is caused by high toxicity of butanol to the production cells, and therefore, understanding the mechanisms by which clostridia react to butanol shock is of key importance. Detailed analyses of transcriptome data obtained after butanol shock, and their comparison with data from standard ABE fermentations resulted in new findings while they confirmed expected population responses as well. Although butanol shock resulted in upregulation of heat shock protein genes, their regulation is probably different than was assumed based on standard ABE fermentation transcriptome data. While glucose uptake, glycolysis and acidogenesis genes were downregulated after butanol shock, solventogenesis genes were upregulated. Cyclopropanation of fatty acids and formation of plasmalogens seem to be significant processes involved in cell membrane stabilization in the presence of butanol. Surprisingly, one of three identified Agr quorum sensing system genes was upregulated. Upregulation of several putative butanol efflux pumps was described after butanol addition and a large putative polyketide gene cluster was found, the transcription of which seemed to be dependent on the concentration of butanol.

Název v anglickém jazyce

Deeper below the surface – transcriptional changes of selected Clostridium beijerinckii NRRL B-598 genes induced by butanol shock

Popis výsledku anglicky

The main bottleneck in the return of industrial butanol production from renewable feedstock, by acetone-butanol-ethanol (ABE) fermentation by clostridia, such as Clostridium beijerinckii, is the low final butanol concentration. The problem is caused by high toxicity of butanol to the production cells, and therefore, understanding the mechanisms by which clostridia react to butanol shock is of key importance. Detailed analyses of transcriptome data obtained after butanol shock, and their comparison with data from standard ABE fermentations resulted in new findings while they confirmed expected population responses as well. Although butanol shock resulted in upregulation of heat shock protein genes, their regulation is probably different than was assumed based on standard ABE fermentation transcriptome data. While glucose uptake, glycolysis and acidogenesis genes were downregulated after butanol shock, solventogenesis genes were upregulated. Cyclopropanation of fatty acids and formation of plasmalogens seem to be significant processes involved in cell membrane stabilization in the presence of butanol. Surprisingly, one of three identified Agr quorum sensing system genes was upregulated. Upregulation of several putative butanol efflux pumps was described after butanol addition and a large putative polyketide gene cluster was found, the transcription of which seemed to be dependent on the concentration of butanol.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA17-00551S" target="_blank" >GA17-00551S: Vztah mezi efluxem butanolu a tolerancí k butanolu u klostridií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MicrobiologyOpen

ISSN

2045-8827

e-ISSN

—

Svazek periodika

00

Číslo periodika v rámci svazku

2020-12-14

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

1-14

Kód UT WoS článku

000598372300001

EID výsledku v databázi Scopus

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