Potential functional capacity of a non-model organism Aneurinibacillus species H1

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F21%3APU141070" target="_blank" >RIV/00216305:26220/21:PU141070 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Potential functional capacity of a non-model organism Aneurinibacillus species H1

Popis výsledku v původním jazyce

Polyhydroxyalkanoates (PHA) are polyesters similar to industrially processed synthetic polymers. Aneurinibacillus species H1 belongs to thermophilic gram-positive bacteria capable of accumulating the PHA in a high amount under certain conditions. Whereas the bacterium´s genetic information has not been studied for its ability to accumulate PHA yet, we bring the first genome assembly along with insight into its functional properties. The genome was assembled using sequencing data obtained from Oxford Nanopore MinION and Illumina MiSeq platforms. In the first step, the initial assembly from Nanopore reads was created. Subsequently, trimmed Illumina reads were mapped to the initial assembly with the result of final assembly. The final assembly was rearranged according to the replication origin (oriC) in a way that the DnaA gene is the first gene. To reveal potential functional capacity of the genome, we analysed particular protein coding genes and divide them according to clusters of orthologous genes (C

Název v anglickém jazyce

Potential functional capacity of a non-model organism Aneurinibacillus species H1

Popis výsledku anglicky

Polyhydroxyalkanoates (PHA) are polyesters similar to industrially processed synthetic polymers. Aneurinibacillus species H1 belongs to thermophilic gram-positive bacteria capable of accumulating the PHA in a high amount under certain conditions. Whereas the bacterium´s genetic information has not been studied for its ability to accumulate PHA yet, we bring the first genome assembly along with insight into its functional properties. The genome was assembled using sequencing data obtained from Oxford Nanopore MinION and Illumina MiSeq platforms. In the first step, the initial assembly from Nanopore reads was created. Subsequently, trimmed Illumina reads were mapped to the initial assembly with the result of final assembly. The final assembly was rearranged according to the replication origin (oriC) in a way that the DnaA gene is the first gene. To reveal potential functional capacity of the genome, we analysed particular protein coding genes and divide them according to clusters of orthologous genes (C

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů