Nanoporous Enzyme-Based Impedimetric Biosensor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F22%3APU145005" target="_blank" >RIV/00216305:26220/22:PU145005 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2022_sbornik_1_v2.pdf" target="_blank" >https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2022_sbornik_1_v2.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Nanoporous Enzyme-Based Impedimetric Biosensor

Popis výsledku v původním jazyce

The ordered nanopores surfaces has been studied in the field of biosensors for the detection of affinity interactions such as antigen-antibody or nucleic acid hybridization. Nanopores are frequently fabricated by anodic oxidation of aluminium. This method is practically simple method of highly ordered pore formation, but is not suitable for massive production. The principle of detection is usually based on the degree of pore blockage by biomolecular interaction that allows a sensitive electrochemical response. Here we show the development and characterization of the nanopore-based enzymatic biosensor for the detection of analytes. We performed and tested the concept of the simple and versatile method of nanopores formation on the electrode using non-conductive polystyrene nanoparticles. The variability in pore size was modulated by nanoparticles diameter (40 and 80 nm). We measured response of our designed biosensor using electrochemical impedance spectroscopy in presence of redox mediator at low pote

Název v anglickém jazyce

Nanoporous Enzyme-Based Impedimetric Biosensor

Popis výsledku anglicky

The ordered nanopores surfaces has been studied in the field of biosensors for the detection of affinity interactions such as antigen-antibody or nucleic acid hybridization. Nanopores are frequently fabricated by anodic oxidation of aluminium. This method is practically simple method of highly ordered pore formation, but is not suitable for massive production. The principle of detection is usually based on the degree of pore blockage by biomolecular interaction that allows a sensitive electrochemical response. Here we show the development and characterization of the nanopore-based enzymatic biosensor for the detection of analytes. We performed and tested the concept of the simple and versatile method of nanopores formation on the electrode using non-conductive polystyrene nanoparticles. The variability in pore size was modulated by nanoparticles diameter (40 and 80 nm). We measured response of our designed biosensor using electrochemical impedance spectroscopy in presence of redox mediator at low pote

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů