Enzyme-Based Impedimetric Biosensor dotted with gold nanoparticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F24%3APU151867" target="_blank" >RIV/00216305:26220/24:PU151867 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2024_sbornik_2.pdf" target="_blank" >https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2024_sbornik_2.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Enzyme-Based Impedimetric Biosensor dotted with gold nanoparticles

Popis výsledku v původním jazyce

This research delves into the realm of biosensor improvement through the utilization of gold nanoparticles (Au NPs). The primary objective is to assess the impact of different-sized Au NPs on sensor performance, specifically investigating whether 100 nm or 20 nm nanoparticles prove more favourable to enhancement. Moreover, we aim to inspect the biosensor's response to varied concentrations of Au NPs, unravelling the involved collaboration between nanoparticle size, concentration, and overall sensor properties. This modification of commercial electrodes with Au NPs, could be way for enhancing surface area and enzyme immobilization. Notably, the investigation also explores the potential drawbacks associated with increasing nanoparticle concentration and offers insights into optimizing biosensor design. It has been observed that while 20 nm Au NPs slightly decreased impedance values at higher glucose concentrations, 100 nm Au NPs, conversely, exhibited an increase in capacitive behaviour. Equally crucial is the parameter chosen for constructing the calibration curve. From impedance values at low frequencies of alternating voltage, such as 2 Hz, a lower Limit of Detection (LOD) is obtained. However, the analysis of Rct in the case of 20 nm Au NPs reveals a broader range of glucose concentrations falling within the calibration area. Through a comprehensive analysis of electrochemical behaviour, impedance, and charge transfer resistance, we endeavour to provide contributions to the improvement of biosensor technologies.

Název v anglickém jazyce

Enzyme-Based Impedimetric Biosensor dotted with gold nanoparticles

Popis výsledku anglicky

This research delves into the realm of biosensor improvement through the utilization of gold nanoparticles (Au NPs). The primary objective is to assess the impact of different-sized Au NPs on sensor performance, specifically investigating whether 100 nm or 20 nm nanoparticles prove more favourable to enhancement. Moreover, we aim to inspect the biosensor's response to varied concentrations of Au NPs, unravelling the involved collaboration between nanoparticle size, concentration, and overall sensor properties. This modification of commercial electrodes with Au NPs, could be way for enhancing surface area and enzyme immobilization. Notably, the investigation also explores the potential drawbacks associated with increasing nanoparticle concentration and offers insights into optimizing biosensor design. It has been observed that while 20 nm Au NPs slightly decreased impedance values at higher glucose concentrations, 100 nm Au NPs, conversely, exhibited an increase in capacitive behaviour. Equally crucial is the parameter chosen for constructing the calibration curve. From impedance values at low frequencies of alternating voltage, such as 2 Hz, a lower Limit of Detection (LOD) is obtained. However, the analysis of Rct in the case of 20 nm Au NPs reveals a broader range of glucose concentrations falling within the calibration area. Through a comprehensive analysis of electrochemical behaviour, impedance, and charge transfer resistance, we endeavour to provide contributions to the improvement of biosensor technologies.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings II of the 30 th Conference STUDENT EEICT 2024

ISBN

978-80-214-6230-4

ISSN

2788-1334

e-ISSN

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Počet stran výsledku

5

Strana od-do

202-206

Název nakladatele

Brno University of Technology, Faculty of Electrical Engineering

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

23. 4. 2024

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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