Diagnostic Performance of an Automated Robot for MALDI Target Preparation in Microbial Identification

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F24%3APU152009" target="_blank" >RIV/00216305:26220/24:PU152009 - isvavai.cz</a>

Výsledek na webu

<a href="https://journals.asm.org/doi/10.1128/jcm.00434-24" target="_blank" >https://journals.asm.org/doi/10.1128/jcm.00434-24</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/jcm.00434-24" target="_blank" >10.1128/jcm.00434-24</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Diagnostic Performance of an Automated Robot for MALDI Target Preparation in Microbial Identification

Popis výsledku v původním jazyce

The MBT Pathfinder® is an automated colony-picking robot designed for efficient sample preparation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. This article presents results from three key experiments evaluating the instrument's performance in conjunction with MALDI Biotyper® instrument. The method comparison experiment assessed its clinical performance, demonstrating comparable results with Gram-positive, Gram-negative, and anaerobic bacteria (scores larger than 2.00) and superior performance over simple direct yeast transfer (score: 1.80) when compared to samples prepared manually. The repeatability experiment confirmed consistent performance over multiple days and labs (average log score: 2.12, std. deviation: 0.59). The challenge panel experiment showcased its consistent and accurate performance across various samples and settings, yielding average scores between 1.76 and 2.19. These findings underline the MBT Pathfinder® as a reliable and efficient tool for MALDI-TOF mass spectrometry sample preparation in clinical and research applications.

Název v anglickém jazyce

Diagnostic Performance of an Automated Robot for MALDI Target Preparation in Microbial Identification

Popis výsledku anglicky

The MBT Pathfinder® is an automated colony-picking robot designed for efficient sample preparation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. This article presents results from three key experiments evaluating the instrument's performance in conjunction with MALDI Biotyper® instrument. The method comparison experiment assessed its clinical performance, demonstrating comparable results with Gram-positive, Gram-negative, and anaerobic bacteria (scores larger than 2.00) and superior performance over simple direct yeast transfer (score: 1.80) when compared to samples prepared manually. The repeatability experiment confirmed consistent performance over multiple days and labs (average log score: 2.12, std. deviation: 0.59). The challenge panel experiment showcased its consistent and accurate performance across various samples and settings, yielding average scores between 1.76 and 2.19. These findings underline the MBT Pathfinder® as a reliable and efficient tool for MALDI-TOF mass spectrometry sample preparation in clinical and research applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30401 - Health-related biotechnology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF CLINICAL MICROBIOLOGY

ISSN

0095-1137

e-ISSN

1098-660X

Svazek periodika

neuveden

Číslo periodika v rámci svazku

09-2024

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

1-12

Kód UT WoS článku

001315322400001

EID výsledku v databázi Scopus

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