Expression profiles of sRNA genes in Caldimonas thermodepolymerans

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F26%3A0199251" target="_blank" >RIV/00216305:26220/26:0199251 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2025_sbornik_2.pdf" target="_blank" >https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2025_sbornik_2.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.13164/eeict.2025.140" target="_blank" >10.13164/eeict.2025.140</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expression profiles of sRNA genes in Caldimonas thermodepolymerans

Popis výsledku v původním jazyce

Regulation of gene expression represents a complex process that monitors the requirements of cells for specific gene products and adjusts their production accordingly. Each stage of gene expression employs different regulatory mechanisms. Here, we pay attention to post-transcriptional regulation by non-coding genomic elements - small RNAs (sRNAs). The aim is to address the problem of missing structural annotations of sRNA genes that play a key role in the rapid response of bacterial cells to stress conditions. For these purposes, we analyse the transcriptomic data of Caldimonas thermodepolymerans DSM 15344T, a promising adept for industrial biotechnology applications, especially for producing polyhydroxyalkanoates (PHA). Analysis of the RNA-Seq data results in a list of predicted sRNAs. Furthermore, the association of these regulatory elements with specific genes is indicated using loci information and sRNA gene co-expression analysis. Finally, sRNA expression analysis was performed to reveal the relationship of sRNA regulation to genes involved in PHA metabolism.

Název v anglickém jazyce

Expression profiles of sRNA genes in Caldimonas thermodepolymerans

Popis výsledku anglicky

Regulation of gene expression represents a complex process that monitors the requirements of cells for specific gene products and adjusts their production accordingly. Each stage of gene expression employs different regulatory mechanisms. Here, we pay attention to post-transcriptional regulation by non-coding genomic elements - small RNAs (sRNAs). The aim is to address the problem of missing structural annotations of sRNA genes that play a key role in the rapid response of bacterial cells to stress conditions. For these purposes, we analyse the transcriptomic data of Caldimonas thermodepolymerans DSM 15344T, a promising adept for industrial biotechnology applications, especially for producing polyhydroxyalkanoates (PHA). Analysis of the RNA-Seq data results in a list of predicted sRNAs. Furthermore, the association of these regulatory elements with specific genes is indicated using loci information and sRNA gene co-expression analysis. Finally, sRNA expression analysis was performed to reveal the relationship of sRNA regulation to genes involved in PHA metabolism.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings II of the 31th Conference STUDENT EEICT 2025

ISBN

978-80-214-6320-2

ISSN

—

e-ISSN

—

Počet stran výsledku

246

Strana od-do

140-144

Název nakladatele

Brno University of Technology

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

29. 4. 2025

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—