Development and testing of a sampling device for the detection of aerosolized pathogens in indoor environments

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F26%3A0199647" target="_blank" >RIV/00216305:26220/26:0199647 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0039914025016364" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0039914025016364</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2025.129145" target="_blank" >10.1016/j.talanta.2025.129145</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development and testing of a sampling device for the detection of aerosolized pathogens in indoor environments

Popis výsledku v původním jazyce

Effective airborne pathogen surveillance is essential for controlling the spread of respiratory infections in public spaces. A simple and effective sampling device, together with the validated sampling scheme and subsequent downstream analysis is needed. We developed and tested wet and dry air sampling concepts to efficiently capture aerosolized model microorganisms. Using Bacillus subtilis and feline infectious peritonitis virus (FIPV, a model for SARS-CoV-2), we evaluated capture efficiency via qPCR-based detection. The dry sampling concept based on the utilization of the intrinsic charge of aerosolized particles demonstrated superior efficiency. After determination of the correct electric field intensity and sampling time, the devices proved their routine applicability by successful sampling in lecture halls and a supermarket, where a higher fluctuation of people was expected. These findings have led to the development of a compact, mobile, and practical air sampling system for real-time pathogen monitoring, offering a valuable tool for epidemiological surveillance and infection control in highoccupancy environments.

Název v anglickém jazyce

Development and testing of a sampling device for the detection of aerosolized pathogens in indoor environments

Popis výsledku anglicky

Effective airborne pathogen surveillance is essential for controlling the spread of respiratory infections in public spaces. A simple and effective sampling device, together with the validated sampling scheme and subsequent downstream analysis is needed. We developed and tested wet and dry air sampling concepts to efficiently capture aerosolized model microorganisms. Using Bacillus subtilis and feline infectious peritonitis virus (FIPV, a model for SARS-CoV-2), we evaluated capture efficiency via qPCR-based detection. The dry sampling concept based on the utilization of the intrinsic charge of aerosolized particles demonstrated superior efficiency. After determination of the correct electric field intensity and sampling time, the devices proved their routine applicability by successful sampling in lecture halls and a supermarket, where a higher fluctuation of people was expected. These findings have led to the development of a compact, mobile, and practical air sampling system for real-time pathogen monitoring, offering a valuable tool for epidemiological surveillance and infection control in highoccupancy environments.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

30302 - Epidemiology

Projekt

<a href="/cs/project/VI04000071" target="_blank" >VI04000071: Systém pro vzorkování a detekci koronaviru a dalších původců respiračních onemocnění ze vzduchu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2026

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

TALANTA

ISSN

0039-9140

e-ISSN

1873-3573

Svazek periodika

300

Číslo periodika v rámci svazku

129145

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

1-12

Kód UT WoS článku

001630510500001

EID výsledku v databázi Scopus

2-s2.0-105023413885