Low-power microfluidic RT-LAMP system with real-time fluorescence detection for portable nucleic acid testing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F26%3A0200011" target="_blank" >RIV/00216305:26220/26:0200011 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00604-025-07680-2" target="_blank" >https://link.springer.com/article/10.1007/s00604-025-07680-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00604-025-07680-2" target="_blank" >10.1007/s00604-025-07680-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Low-power microfluidic RT-LAMP system with real-time fluorescence detection for portable nucleic acid testing

Popis výsledku v původním jazyce

A microfluidic reverse transcription loop-mediated isothermal amplification (RT-LAMP) platform is presented featuring integrated real-time fluorescence detection and optimized thermal control. Magnetic bead-based RNA extraction was paired with a thermally insulated reaction chip operated under vacuum to suppress heat loss. Additional energy-saving strategies, including reduced wire cross-section and low-emissivity surface treatment, reduced total power consumption from (2.00 +/- 0.08) W to (0.29 +/- 0.01) W, corresponding to an 85.5 % reduction. Amplification was completed within 20 min, and positive fluorescence signals were detected in less than 11 min. Although LAMP reagents remain more expensive than those used in PCR, the substantial energy savings and system simplification demonstrated here support practical battery-powered operation. The RT-LAMP chemistry used in this system matched a previously validated configuration, which achieved a limit of detection of 256 RNA copies and a signal slope of (- 1.70 +/- 0.20) dec.min(-)1. These parameters remained consistent under the current setup, as power consumption did not influence detection sensitivity provided the reaction temperature was maintained. The platform enables low-power, real-time nucleic acid detection suited for decentralized diagnostics, forensic screening, and environmental monitoring.

Název v anglickém jazyce

Low-power microfluidic RT-LAMP system with real-time fluorescence detection for portable nucleic acid testing

Popis výsledku anglicky

A microfluidic reverse transcription loop-mediated isothermal amplification (RT-LAMP) platform is presented featuring integrated real-time fluorescence detection and optimized thermal control. Magnetic bead-based RNA extraction was paired with a thermally insulated reaction chip operated under vacuum to suppress heat loss. Additional energy-saving strategies, including reduced wire cross-section and low-emissivity surface treatment, reduced total power consumption from (2.00 +/- 0.08) W to (0.29 +/- 0.01) W, corresponding to an 85.5 % reduction. Amplification was completed within 20 min, and positive fluorescence signals were detected in less than 11 min. Although LAMP reagents remain more expensive than those used in PCR, the substantial energy savings and system simplification demonstrated here support practical battery-powered operation. The RT-LAMP chemistry used in this system matched a previously validated configuration, which achieved a limit of detection of 256 RNA copies and a signal slope of (- 1.70 +/- 0.20) dec.min(-)1. These parameters remained consistent under the current setup, as power consumption did not influence detection sensitivity provided the reaction temperature was maintained. The platform enables low-power, real-time nucleic acid detection suited for decentralized diagnostics, forensic screening, and environmental monitoring.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/TN02000017" target="_blank" >TN02000017: Národní Centrum Biotechnologií ve Veterinární Medicíně</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MICROCHIMICA ACTA

ISSN

0026-3672

e-ISSN

1436-5073

Svazek periodika

1

Číslo periodika v rámci svazku

193

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

001644808100001

EID výsledku v databázi Scopus

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