Využití HPLC a GPC pro charakterizaci produktů degradace biokompatibilních polymerů

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Use of HPLC and GPC in the characterization of biocompatible polymer degradation products

Popis výsledku v původním jazyce

New non-toxic biodegradable biocompatible amphiphilic end-functionalized hydrogel based on the thermosensitive triblock macromonomer gained from hydrophilic poly(ethylene glycol) and hydrophobic copolymer of poly(lactic acid-co-glycolic acid) modified byitaconic acid (PEG-ITA-PLGA-ITA-PEG) was synthesized and analyzed using liquid chromatography methods. GPC analysis was used for the determination of an average molecular weight and polymer distribution of the single steps of the biodegradable PEG-ITA-PLGA-ITA-PEG macromonomer synthesis. On the other hand, the RP-HPLC method was optimized for the determination of the degradation products of PEG-ITA-PLGA-ITA-PEG copolymer. The peaks separation was influenced by a mobile phase composition, pH, injectionvolume and a sample solvent. The best separation of the glycolic, diglycolic and lactic acid was obtained for the mobile phase consisted of binary solvent mixture of acetonitrile : phosphate buffer having ratio of 5:95 (v/v) at pH = 3 and

Název v anglickém jazyce

Use of HPLC and GPC in the characterization of biocompatible polymer degradation products

Popis výsledku anglicky

New non-toxic biodegradable biocompatible amphiphilic end-functionalized hydrogel based on the thermosensitive triblock macromonomer gained from hydrophilic poly(ethylene glycol) and hydrophobic copolymer of poly(lactic acid-co-glycolic acid) modified byitaconic acid (PEG-ITA-PLGA-ITA-PEG) was synthesized and analyzed using liquid chromatography methods. GPC analysis was used for the determination of an average molecular weight and polymer distribution of the single steps of the biodegradable PEG-ITA-PLGA-ITA-PEG macromonomer synthesis. On the other hand, the RP-HPLC method was optimized for the determination of the degradation products of PEG-ITA-PLGA-ITA-PEG copolymer. The peaks separation was influenced by a mobile phase composition, pH, injectionvolume and a sample solvent. The best separation of the glycolic, diglycolic and lactic acid was obtained for the mobile phase consisted of binary solvent mixture of acetonitrile : phosphate buffer having ratio of 5:95 (v/v) at pH = 3 and

Druh

A - Audiovizuální tvorba

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

ISBN

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Místo vydání

Riva Del Garda, Italy

Název nakladatele resp. objednatele

Resaerch Institute for Chromatography

Verze

1

Identifikační číslo nosiče

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