Application of magnetic carriers for whole DNA isolation and detection of probiotic Lactobacillus gasseri K7 strain in complex samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F09%3APU83416" target="_blank" >RIV/00216305:26310/09:PU83416 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Application of magnetic carriers for whole DNA isolation and detection of probiotic Lactobacillus gasseri K7 strain in complex samples

Popis výsledku v původním jazyce

The importance of lactic acid bacteria (LAB) for dairy industry is well known. Increasing interest has been focused on some LAB strains that are proposed to be probiotic. Lactobacillus gasseri K7 is a probiotic strain, producer of gassericins K7 A and B1, which was tested in vivo in mice. Falsely negative results can occur in PCR identification of target cells due to the presence of extracellular PCR inhibitors in tested samples - mouse faeces. The problem can be solved by solid phase systems adsorbinggenomic DNA. Carboxyl group-containing magnetic nonporous poly(2-hydroxyethyl methacrylate-co-glycidyl dimethylmethacrylate) (P(HEMA-co-GMA) were used for the isolation of whole DNA from mouse faeces. These microspheres reversibly bind DNA in the presence of high concentrations of poly(ethylene glycol) (PEG 6000) and sodium chloride2. The release of adsorbed DNA from the microsphereses was optimalised using a low-ionic-strength TE buffer of different pH and temperature. The presence of t

Název v anglickém jazyce

Application of magnetic carriers for whole DNA isolation and detection of probiotic Lactobacillus gasseri K7 strain in complex samples

Popis výsledku anglicky

The importance of lactic acid bacteria (LAB) for dairy industry is well known. Increasing interest has been focused on some LAB strains that are proposed to be probiotic. Lactobacillus gasseri K7 is a probiotic strain, producer of gassericins K7 A and B1, which was tested in vivo in mice. Falsely negative results can occur in PCR identification of target cells due to the presence of extracellular PCR inhibitors in tested samples - mouse faeces. The problem can be solved by solid phase systems adsorbinggenomic DNA. Carboxyl group-containing magnetic nonporous poly(2-hydroxyethyl methacrylate-co-glycidyl dimethylmethacrylate) (P(HEMA-co-GMA) were used for the isolation of whole DNA from mouse faeces. These microspheres reversibly bind DNA in the presence of high concentrations of poly(ethylene glycol) (PEG 6000) and sodium chloride2. The release of adsorbed DNA from the microsphereses was optimalised using a low-ionic-strength TE buffer of different pH and temperature. The presence of t

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/2B08070" target="_blank" >2B08070: Vývoj nového moderního biotechnologického postupu umožňujícího účinnější způsob využívání odpadních vod z mlékárenského průmyslu jako materiálového zdroje pro výrobu vodíku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Gastro jenterologia

ISSN

1727-7906

e-ISSN

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Svazek periodika

11

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

RU - Ruská federace

Počet stran výsledku

1

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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