The use of enzymes contained in the washing powders to the preparation of the crude lyzates of Lactobacillus cells for magnetic DNA isolation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F11%3APU94303" target="_blank" >RIV/00216305:26310/11:PU94303 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The use of enzymes contained in the washing powders to the preparation of the crude lyzates of Lactobacillus cells for magnetic DNA isolation

Popis výsledku v původním jazyce

The preparation of crude lysates of bacterial cells is relatively expensive procedure because of the enzymes are used during the procedure. This problem can be solved by using of the washing powders for the cell lysis. The aim of our work was the isolation of DNA from the Lactobacillus at quality for the PCRs and to test whether the DNA isolated by the magnetic microsperes (P(HEMA-co-GMA) is appropriate to run the PCR with. We tried to find out if the composition of the washing powders is suitable for disruption of the cell wall instead of lysosyme.

Název v anglickém jazyce

The use of enzymes contained in the washing powders to the preparation of the crude lyzates of Lactobacillus cells for magnetic DNA isolation

Popis výsledku anglicky

The preparation of crude lysates of bacterial cells is relatively expensive procedure because of the enzymes are used during the procedure. This problem can be solved by using of the washing powders for the cell lysis. The aim of our work was the isolation of DNA from the Lactobacillus at quality for the PCRs and to test whether the DNA isolated by the magnetic microsperes (P(HEMA-co-GMA) is appropriate to run the PCR with. We tried to find out if the composition of the washing powders is suitable for disruption of the cell wall instead of lysosyme.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů