Analysis of Vitamin D in feed by UPLC-MS/MS
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F13%3APU105791" target="_blank" >RIV/00216305:26310/13:PU105791 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Analysis of Vitamin D in feed by UPLC-MS/MS
Popis výsledku v původním jazyce
Various forms of vitamin D, namely cholecalciferol (vitamin D3), ergocalciferol (vitamin D2) and 25-hydroxycholecalciferol, are included in the European Union Register of Feed Additives. Maximum limits for these compounds have been established and vary depending on the feed [1, 2]. The simultaneous use of different forms of vitamin D is prohibited or strictly directed. Central Institute for Supervising and Testing in Agriculture inspects the animal feedstuffs, premixtures and mineral feeds for various parameters including the content of cholecalciferol. Method for determination of vitamin D based on BS EN 12821:2009 [3] has been validated. Sample preparation is based on liquid-liquid extraction after alkaline saponification and determination is carriedout by ultra-performance liquid chromatography coupled to tandem mass spectrometry using positive electrospray ionization (UPLC-MS/MS). Deuterated cholecalciferol is used as internal standard for the quantification of cholecalciferol in
Název v anglickém jazyce
Analysis of Vitamin D in feed by UPLC-MS/MS
Popis výsledku anglicky
Various forms of vitamin D, namely cholecalciferol (vitamin D3), ergocalciferol (vitamin D2) and 25-hydroxycholecalciferol, are included in the European Union Register of Feed Additives. Maximum limits for these compounds have been established and vary depending on the feed [1, 2]. The simultaneous use of different forms of vitamin D is prohibited or strictly directed. Central Institute for Supervising and Testing in Agriculture inspects the animal feedstuffs, premixtures and mineral feeds for various parameters including the content of cholecalciferol. Method for determination of vitamin D based on BS EN 12821:2009 [3] has been validated. Sample preparation is based on liquid-liquid extraction after alkaline saponification and determination is carriedout by ultra-performance liquid chromatography coupled to tandem mass spectrometry using positive electrospray ionization (UPLC-MS/MS). Deuterated cholecalciferol is used as internal standard for the quantification of cholecalciferol in
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů