The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F14%3APU109810" target="_blank" >RIV/00216305:26310/14:PU109810 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR

Popis výsledku v původním jazyce

Isolation of DNA is an important step in the molecular diagnostics of microorganisms and viruses, medical diagnosis, etc. Different magnetic nano- and microparticles have been used as a solid support for DNA isolation. Magnetic nano- and microparticles usually consist of a number of components: supermagnetic core, protective coating and the surface functionality. Iron oxides and some substances used to stabilize magnetic cores reduce biocompatibility of the proposed carriers, therefore perfectly cover core prerequisite for their application in molecular diagnostics. Magnetic cores are covered with a variety of functionalized low-or high-molecular compounds (polymer layer). The work was focused on the estimation of influence of microparticles with magnetite core on the amplification of DNA. A set of 8 types of hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) ? P(HEMA-co-GMA) microparticles, 6 types of poly(glycidyl methacrylate) (PGMA) microparticles and

Název v anglickém jazyce

The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR

Popis výsledku anglicky

Isolation of DNA is an important step in the molecular diagnostics of microorganisms and viruses, medical diagnosis, etc. Different magnetic nano- and microparticles have been used as a solid support for DNA isolation. Magnetic nano- and microparticles usually consist of a number of components: supermagnetic core, protective coating and the surface functionality. Iron oxides and some substances used to stabilize magnetic cores reduce biocompatibility of the proposed carriers, therefore perfectly cover core prerequisite for their application in molecular diagnostics. Magnetic cores are covered with a variety of functionalized low-or high-molecular compounds (polymer layer). The work was focused on the estimation of influence of microparticles with magnetite core on the amplification of DNA. A set of 8 types of hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) ? P(HEMA-co-GMA) microparticles, 6 types of poly(glycidyl methacrylate) (PGMA) microparticles and

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP13-32840P" target="_blank" >GP13-32840P: Vývoj metody pro studium enkapsulace magnetických nano- a mikročástic pomocí polymerázové řetězové reakce v reálném čase</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů