SOLID - PHASE DNA EXTRACTION USING VARIOUS TYPES OF MAGNETIC MICROPARTICLES

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU115356" target="_blank" >RIV/00216305:26310/15:PU115356 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

SOLID - PHASE DNA EXTRACTION USING VARIOUS TYPES OF MAGNETIC MICROPARTICLES

Popis výsledku v původním jazyce

Isolation of DNA is an important step in the molecular diagnostics of microorganisms. A high quality of isolated DNA is necessary for DNA amplification by the polymerase chain reaction. The conventional DNA isolation using phenol-chloroform extraction and DNA precipitation in ethanol is time-consuming and requires the use of toxic phenol. Magnetic separation techniques using magnetic solid particles are one of modern methods to speed up the nucleic acids isolation. The aim of of this work was to use 3 different types of magnetic microparticles, prepared by Horák, for solid - phase DNA extraction. The amounts of DNA in separation mixtures were measured by using UV-VIS spectrophotometry. The first experimental conditions were tested on chicken erythrocytes DNA. Phosphate buffer (pH 7, 7.6 and 8) was used for adsorption of magnetic particles. It was shown that approximately one half of DNA was adsorbed to the particles. The elution conditions of DNA were also optimized. Secondly bacterial

Název v anglickém jazyce

SOLID - PHASE DNA EXTRACTION USING VARIOUS TYPES OF MAGNETIC MICROPARTICLES

Popis výsledku anglicky

Isolation of DNA is an important step in the molecular diagnostics of microorganisms. A high quality of isolated DNA is necessary for DNA amplification by the polymerase chain reaction. The conventional DNA isolation using phenol-chloroform extraction and DNA precipitation in ethanol is time-consuming and requires the use of toxic phenol. Magnetic separation techniques using magnetic solid particles are one of modern methods to speed up the nucleic acids isolation. The aim of of this work was to use 3 different types of magnetic microparticles, prepared by Horák, for solid - phase DNA extraction. The amounts of DNA in separation mixtures were measured by using UV-VIS spectrophotometry. The first experimental conditions were tested on chicken erythrocytes DNA. Phosphate buffer (pH 7, 7.6 and 8) was used for adsorption of magnetic particles. It was shown that approximately one half of DNA was adsorbed to the particles. The elution conditions of DNA were also optimized. Secondly bacterial

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů