Comparison of magnetic nano- and microparticles application for DNA isolation from real samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU117520" target="_blank" >RIV/00216305:26310/15:PU117520 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of magnetic nano- and microparticles application for DNA isolation from real samples

Popis výsledku v původním jazyce

In the past decade, magnetic nanoparticles and mickroparticles have been widely employed for numerous biomedical and biological applications. The aim of this work was to compare the application of poly (L-lysine) (PLL) modified iron oxide nanoparticles and hydrophilic magnetic non-porus poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate- P(HEMA-co-GMA) microsphere for DNA isolation from food supplements. Conventional phenol extraction procedure was used as control. Phosphate buffer (pH 7,8) and TE buffer containing 10mM KCl (pH 9.0) and phosphate buffer (pH 8.0) were used fot DNA adsorption /elution in the case of poly (L-lysine) (PLL) modified iron oxide nanoparticles? mixture 16% PEG 6,000 and 2.0 M NaCl and TE buffer were used for DNA adsorption/elution in case of P(HEMA-co-GMA) microsphere. Method was applied for DNA isolation from following food supplements: Linex?forte, Laktobacily forte, Pangamin and Lactomax. Quantity of extracted DNA was checked spectrofotometrically an

Název v anglickém jazyce

Comparison of magnetic nano- and microparticles application for DNA isolation from real samples

Popis výsledku anglicky

In the past decade, magnetic nanoparticles and mickroparticles have been widely employed for numerous biomedical and biological applications. The aim of this work was to compare the application of poly (L-lysine) (PLL) modified iron oxide nanoparticles and hydrophilic magnetic non-porus poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate- P(HEMA-co-GMA) microsphere for DNA isolation from food supplements. Conventional phenol extraction procedure was used as control. Phosphate buffer (pH 7,8) and TE buffer containing 10mM KCl (pH 9.0) and phosphate buffer (pH 8.0) were used fot DNA adsorption /elution in the case of poly (L-lysine) (PLL) modified iron oxide nanoparticles? mixture 16% PEG 6,000 and 2.0 M NaCl and TE buffer were used for DNA adsorption/elution in case of P(HEMA-co-GMA) microsphere. Method was applied for DNA isolation from following food supplements: Linex?forte, Laktobacily forte, Pangamin and Lactomax. Quantity of extracted DNA was checked spectrofotometrically an

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů