Steady-State Fluorescence Spectroscopy Techniques in Determination of Phospholipids Vesicles Properties

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU119104" target="_blank" >RIV/00216305:26310/15:PU119104 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Steady-State Fluorescence Spectroscopy Techniques in Determination of Phospholipids Vesicles Properties

Popis výsledku v původním jazyce

Steady-state fluorescence techniques represent a powerful tool for the determination of great variety of phospholipids vesicles (liposome) properties. Phase transition temperature is one of the most important characteristics of liposomes. It is commonlydetermined by means of calorimetric technics. It can be also obtained by analyzing of laurdan emission spectra at different temperatures. The degree of a lipid acyl chain order in liposomes and a membrane fluidity can by studied by means of fluorescenceanisotropy, which measures depolarization of fluorophores excited by polarized light, where the depolarization depends on the membrane fluidity. Diphenylhexatriene is a suitable fluorescence probe for the anisotropy measurements. The aggregation behaviorof lipid molecules can be studied using fluorescence probes such as pyrene or perylene. These fluorescence probes are sensitive to the polarity of the local environment and indicate changes in lipid aggregates character. Fluorescent lipo

Název v anglickém jazyce

Steady-State Fluorescence Spectroscopy Techniques in Determination of Phospholipids Vesicles Properties

Popis výsledku anglicky

Steady-state fluorescence techniques represent a powerful tool for the determination of great variety of phospholipids vesicles (liposome) properties. Phase transition temperature is one of the most important characteristics of liposomes. It is commonlydetermined by means of calorimetric technics. It can be also obtained by analyzing of laurdan emission spectra at different temperatures. The degree of a lipid acyl chain order in liposomes and a membrane fluidity can by studied by means of fluorescenceanisotropy, which measures depolarization of fluorophores excited by polarized light, where the depolarization depends on the membrane fluidity. Diphenylhexatriene is a suitable fluorescence probe for the anisotropy measurements. The aggregation behaviorof lipid molecules can be studied using fluorescence probes such as pyrene or perylene. These fluorescence probes are sensitive to the polarity of the local environment and indicate changes in lipid aggregates character. Fluorescent lipo

Druh

O - Ostatní výsledky

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů