Ecotoxicity Assessment of Cadmium Using Different Life Stages of the Terrestrial Gastropod Helix aspersa aspersa

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Ecotoxicity Assessment of Cadmium Using Different Life Stages of the Terrestrial Gastropod Helix aspersa aspersa

Popis výsledku v původním jazyce

The main aim of this work is to present the possibility of using a cosmopolitan terrestrial organism and its two different life stages for evaluating possible effect of environmental contaminants. The organism used in this study is a pulmonate gastropod Helix aspersa aspersa (syn. Cantareus aspersus or Cornu aspersum), or the brown garden snail in common. For this study, we choosed two different life stages of this snail: eggs and juvenile individuals. The evaluated substance was cadmium chloride (CdCl2). There were three main parts of the study: standard growth inhibition test with juvenile snails, hatching success test with the eggs, embryotoxicity test. In the first case, the juvenile snails were exposed to different concentrations of cadmium (0 – 500 mg/l) and the toxicant was spiked onto a filter paper on the bottom of the breeding boxes. This test lasted for 28 days and the endpoints were: mass and shell diameter. Second, the exposition of eggs was performed in small petri dishes with a filter paper on the bottom and the concentrations of cadmium were the same as in the first case. This test lasted for 14 days and the endpoint was the percentage of successfully hatched snails. In the last case, we observed the effect of cadmium in a low concentration (6mg/l) on the embryos in different time. This test lasted also for 14 days and the endpoints were: size and state of the embryo, morphological and physiological changes. The results showed significant differences between the sensitivity of eggs and juvenile snails and also negative effects of cadmium chloride on the development of the snail embryos. The present study summarizes a scale of different endpoints, which could help for better understanding the effects of environmental toxicants.

Název v anglickém jazyce

Ecotoxicity Assessment of Cadmium Using Different Life Stages of the Terrestrial Gastropod Helix aspersa aspersa

Popis výsledku anglicky

The main aim of this work is to present the possibility of using a cosmopolitan terrestrial organism and its two different life stages for evaluating possible effect of environmental contaminants. The organism used in this study is a pulmonate gastropod Helix aspersa aspersa (syn. Cantareus aspersus or Cornu aspersum), or the brown garden snail in common. For this study, we choosed two different life stages of this snail: eggs and juvenile individuals. The evaluated substance was cadmium chloride (CdCl2). There were three main parts of the study: standard growth inhibition test with juvenile snails, hatching success test with the eggs, embryotoxicity test. In the first case, the juvenile snails were exposed to different concentrations of cadmium (0 – 500 mg/l) and the toxicant was spiked onto a filter paper on the bottom of the breeding boxes. This test lasted for 28 days and the endpoints were: mass and shell diameter. Second, the exposition of eggs was performed in small petri dishes with a filter paper on the bottom and the concentrations of cadmium were the same as in the first case. This test lasted for 14 days and the endpoint was the percentage of successfully hatched snails. In the last case, we observed the effect of cadmium in a low concentration (6mg/l) on the embryos in different time. This test lasted also for 14 days and the endpoints were: size and state of the embryo, morphological and physiological changes. The results showed significant differences between the sensitivity of eggs and juvenile snails and also negative effects of cadmium chloride on the development of the snail embryos. The present study summarizes a scale of different endpoints, which could help for better understanding the effects of environmental toxicants.

Druh

A - Audiovizuální tvorba

CEP obor

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OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

ISBN

978-972-752-228-6

Místo vydání

Porto, Portugalsko

Název nakladatele resp. objednatele

Neuveden

Verze

1

Identifikační číslo nosiče

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