Simultaneous production of lipids and pigments by red yeasts grown on animal fat using the concept of biorefinery

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F17%3APU126991" target="_blank" >RIV/00216305:26310/17:PU126991 - isvavai.cz</a>

Výsledek na webu

<a href="http://biomicroworld2017.org/" target="_blank" >http://biomicroworld2017.org/</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous production of lipids and pigments by red yeasts grown on animal fat using the concept of biorefinery

Popis výsledku v původním jazyce

Characterization of metabolic activity of yeasts in biotechnological applications is an important issue both for routine analysis and trouble-shooting incidences. In this work 12 different strains of red yeasts (Rhodotorula, Sporobolomyces, Cystofilobasidium) were used for screening of growth and metabolic activity. Simultaneous production of lipids and other lipid metabolites was followed. As a carbon substrate waste animal fat and its hydrolysis products were used according to the biorefinery concept. Yeasts were cultivated in medium with particular C/N ratio. Whey was used as alternative nitrogen source. High-throughput micro-cultivation, FTIR spectroscopy and single cell analysis of red yeasts was used for monitoring of growth characteristics and total metabolic activity of yeasts. Simultaneously, chromatographic techniques were used for quantification of production of lipids and fatty acids (GC/FID), sterols, carotenoids and CoQ (LC/MS). Glycerol, lipase and total lipids were measured by enzyme

Název v anglickém jazyce

Simultaneous production of lipids and pigments by red yeasts grown on animal fat using the concept of biorefinery

Popis výsledku anglicky

Characterization of metabolic activity of yeasts in biotechnological applications is an important issue both for routine analysis and trouble-shooting incidences. In this work 12 different strains of red yeasts (Rhodotorula, Sporobolomyces, Cystofilobasidium) were used for screening of growth and metabolic activity. Simultaneous production of lipids and other lipid metabolites was followed. As a carbon substrate waste animal fat and its hydrolysis products were used according to the biorefinery concept. Yeasts were cultivated in medium with particular C/N ratio. Whey was used as alternative nitrogen source. High-throughput micro-cultivation, FTIR spectroscopy and single cell analysis of red yeasts was used for monitoring of growth characteristics and total metabolic activity of yeasts. Simultaneously, chromatographic techniques were used for quantification of production of lipids and fatty acids (GC/FID), sterols, carotenoids and CoQ (LC/MS). Glycerol, lipase and total lipids were measured by enzyme

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů