A simple and efficient procedure for capillary blood spiking with basic and acidic drugs for subsequent dried blood spot analysis.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F19%3APU133546" target="_blank" >RIV/00216305:26310/19:PU133546 - isvavai.cz</a>
Výsledek na webu
<a href="https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=60490652627" target="_blank" >https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=60490652627</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
A simple and efficient procedure for capillary blood spiking with basic and acidic drugs for subsequent dried blood spot analysis.
Popis výsledku v původním jazyce
In the actual contribution, a simple process was developed and optimized for effective IS addition and DBS elution. A group of acidic and/or basic drugs, with different chemical characteristics, was added to the collected capillary blood in different ways and the mixture was transferred onto the DBS collection card (903®Whatman™). Various elution procedures were also examined and are discussed. Direct injection of the eluted DBS sample into CE-UV ensured a rapid and reliable determination of the drugs without the need for any additional pretreatment steps (extraction, preconcentration). The contents of analytes in the dissolved eluates were determined and recoveries >70% were achieved for the optimized elution systems with satisfactory repeatability (RSD <5%).
Název v anglickém jazyce
A simple and efficient procedure for capillary blood spiking with basic and acidic drugs for subsequent dried blood spot analysis.
Popis výsledku anglicky
In the actual contribution, a simple process was developed and optimized for effective IS addition and DBS elution. A group of acidic and/or basic drugs, with different chemical characteristics, was added to the collected capillary blood in different ways and the mixture was transferred onto the DBS collection card (903®Whatman™). Various elution procedures were also examined and are discussed. Direct injection of the eluted DBS sample into CE-UV ensured a rapid and reliable determination of the drugs without the need for any additional pretreatment steps (extraction, preconcentration). The contents of analytes in the dissolved eluates were determined and recoveries >70% were achieved for the optimized elution systems with satisfactory repeatability (RSD <5%).
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů