Interconnection of Waste Chicken Feather Biodegradation and Keratinase and mcl-PHA Production Employing Pseudomonas putida KT2440

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F19%3APU135392" target="_blank" >RIV/00216305:26310/19:PU135392 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.google.cz/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=2ahUKEwj1uN_80N3nAhVTh1wKHTTkDFYQFjACegQIAhAB&url=http%3A%2F%2Fjournals.sbmu.ac.ir%2Fafb%2Farticle%2Fview%2F21429&usg=AOvVaw3eqA2hZUKMTPtos_x6IO_H" target="_blank" >https://www.google.cz/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=2ahUKEwj1uN_80N3nAhVTh1wKHTTkDFYQFjACegQIAhAB&url=http%3A%2F%2Fjournals.sbmu.ac.ir%2Fafb%2Farticle%2Fview%2F21429&usg=AOvVaw3eqA2hZUKMTPtos_x6IO_H</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.22037/afb.v6i1.21429" target="_blank" >10.22037/afb.v6i1.21429</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interconnection of Waste Chicken Feather Biodegradation and Keratinase and mcl-PHA Production Employing Pseudomonas putida KT2440

Popis výsledku v původním jazyce

Background and objective: Waste chicken feather is an important waste product of the poultry processing industry and is annually produced in substantial amounts. Hence, wise management of this waste is desirable. In this work we aimed at feathers biodegradation by a selected bacterial strain capable of utilizing chicken feathers as sole carbon source-Pseudomonas putida KT2440. To utilize feather, the bacterial culture excrete keratinase, which can be easily isolated after biodegradation process and which, therefore, represents an interesting side product of the intended technology. Moreover, bacterial culture of employed for feather degradation is also capable of mcl-PHA accumulation. Materials and methods: Bacterial culture of Pseudomonas putida KT2440 was cultivated in presence of waste chicken feathers as the only carbon source; during the cultivation keratinase activity and biomass growth were monitored. Metabolically active biomass after feather degradation was used for mcl-PHA production. Results and conclusion: During cultivation on waste feathers, bacteria did not accumulate detectable amounts of medium-chain length polyhydroxyalkanoate (mcl-PHA); nevertheless, when metabolically active bacterial cells after feather biodegradation were transferred into nitrogen limited mineral media, a high medium-chain length polyhydroxyalkanoate content of 61% of cell dry weight in microbial cells was reached. The polymer consisted of 3-hydroxyhexanoate (27.2% mol) and 3-hydroxyoctanoate (72.8% mol) monomer units. Therefore, this work demonstrates a possible interconnection of feather biodegradation with keratinase and medium-chain length polyhydroxyalkanoate production.

Název v anglickém jazyce

Interconnection of Waste Chicken Feather Biodegradation and Keratinase and mcl-PHA Production Employing Pseudomonas putida KT2440

Popis výsledku anglicky

Background and objective: Waste chicken feather is an important waste product of the poultry processing industry and is annually produced in substantial amounts. Hence, wise management of this waste is desirable. In this work we aimed at feathers biodegradation by a selected bacterial strain capable of utilizing chicken feathers as sole carbon source-Pseudomonas putida KT2440. To utilize feather, the bacterial culture excrete keratinase, which can be easily isolated after biodegradation process and which, therefore, represents an interesting side product of the intended technology. Moreover, bacterial culture of employed for feather degradation is also capable of mcl-PHA accumulation. Materials and methods: Bacterial culture of Pseudomonas putida KT2440 was cultivated in presence of waste chicken feathers as the only carbon source; during the cultivation keratinase activity and biomass growth were monitored. Metabolically active biomass after feather degradation was used for mcl-PHA production. Results and conclusion: During cultivation on waste feathers, bacteria did not accumulate detectable amounts of medium-chain length polyhydroxyalkanoate (mcl-PHA); nevertheless, when metabolically active bacterial cells after feather biodegradation were transferred into nitrogen limited mineral media, a high medium-chain length polyhydroxyalkanoate content of 61% of cell dry weight in microbial cells was reached. The polymer consisted of 3-hydroxyhexanoate (27.2% mol) and 3-hydroxyoctanoate (72.8% mol) monomer units. Therefore, this work demonstrates a possible interconnection of feather biodegradation with keratinase and medium-chain length polyhydroxyalkanoate production.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21101 - Food and beverages

Projekt

<a href="/cs/project/LO1211" target="_blank" >LO1211: Centrum materiálového výzkumu na FCH VUT v Brně - udržitelnost a rozvoj</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

APPLIED FOOD BIOTECHNOLOGY

ISSN

2345-5357

e-ISSN

—

Svazek periodika

6

Číslo periodika v rámci svazku

2345-5357

Stát vydavatele periodika

IR - Íránská islámská republika

Počet stran výsledku

8

Strana od-do

83-90

Kód UT WoS článku

000460206400009

EID výsledku v databázi Scopus

2-s2.0-85061783685