Approaches of evolutionary engineering for adaptation of Cupriavidus necator H16 to biotechnologically relevant stressors

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Approaches of evolutionary engineering for adaptation of Cupriavidus necator H16 to biotechnologically relevant stressors

Popis výsledku v původním jazyce

Model PHA producer Cupriavidus necator H16 (CCM 3726) was exposed to presence of NaCl causing osmotic stress which represented environmental stress factor, to copper ions representing anthropogenic pollutant and levulinic acid, microbial inhibitor commonly presented in hydrolysates of lignocellulosic biomass. Multiple serial transfers of cell cultures in Erlenmeyer flasks after 48 hours were provided within adaptation experiments for more than 80 passages. Passages have been basically characterized (growth potential, analysis of PHAs) and selected preserved ones were also compared with wild-type strain considering potential of PHAs accumulation (GC-FID), testing of robustness, “fingerprint” of cells using Raman spectroscopy and others.

Název v anglickém jazyce

Approaches of evolutionary engineering for adaptation of Cupriavidus necator H16 to biotechnologically relevant stressors

Popis výsledku anglicky

Model PHA producer Cupriavidus necator H16 (CCM 3726) was exposed to presence of NaCl causing osmotic stress which represented environmental stress factor, to copper ions representing anthropogenic pollutant and levulinic acid, microbial inhibitor commonly presented in hydrolysates of lignocellulosic biomass. Multiple serial transfers of cell cultures in Erlenmeyer flasks after 48 hours were provided within adaptation experiments for more than 80 passages. Passages have been basically characterized (growth potential, analysis of PHAs) and selected preserved ones were also compared with wild-type strain considering potential of PHAs accumulation (GC-FID), testing of robustness, “fingerprint” of cells using Raman spectroscopy and others.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10700 - Other natural sciences

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů