Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F21%3APU140905" target="_blank" >RIV/00216305:26310/21:PU140905 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0960852421000213" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0960852421000213</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.biortech.2021.124683" target="_blank" >10.1016/j.biortech.2021.124683</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil
Popis výsledku v původním jazyce
The aim of this work was to develop a soap-based method for the isolation of poly(3-hydroxybutyrate) from bacterial biomass. The method consisted of adding soap derived from waste cooking oil to a concentrated (25%) biomass suspension, heating and centrifugal separation. Purity above 95% could be achieved with soap:cell dry mass ratios at least 0.125 g/g, making the method comparable to other surfactant-based protocols. Molecular weights M, of products from all experiments were between 350 and 450 kDa, being high enough for future material applications. Addition of hydrochloric acid to the wastewater led to the precipitation of soap and part of non-P3HB cell mass. The resulting precipitate was utilized as a carbon source in biomass production and increased substrate-to-P3HB conversion.
Název v anglickém jazyce
Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil
Popis výsledku anglicky
The aim of this work was to develop a soap-based method for the isolation of poly(3-hydroxybutyrate) from bacterial biomass. The method consisted of adding soap derived from waste cooking oil to a concentrated (25%) biomass suspension, heating and centrifugal separation. Purity above 95% could be achieved with soap:cell dry mass ratios at least 0.125 g/g, making the method comparable to other surfactant-based protocols. Molecular weights M, of products from all experiments were between 350 and 450 kDa, being high enough for future material applications. Addition of hydrochloric acid to the wastewater led to the precipitation of soap and part of non-P3HB cell mass. The resulting precipitate was utilized as a carbon source in biomass production and increased substrate-to-P3HB conversion.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40500 - Other agricultural sciences
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
BIORESOURCE TECHNOLOGY
ISSN
0960-8524
e-ISSN
1873-2976
Svazek periodika
326
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
5
Strana od-do
1-5
Kód UT WoS článku
000620172000007
EID výsledku v databázi Scopus
2-s2.0-85100048494