Comparison between synthetic and natural G4-ligands and the effects of their binding to DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F21%3APU141146" target="_blank" >RIV/00216305:26310/21:PU141146 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison between synthetic and natural G4-ligands and the effects of their binding to DNA

Popis výsledku v původním jazyce

Among local and non-canonical DNA structures are G-rich sequences that can form G-quadruplex (G4) sequencees widespread qmong prokaryotic and eukaryotic kingdoms and are an increasingly used targets for regulatory proteins. There are evidence from more than three decades of research confirm their function in important cell processes such as transcription and replication. G4s have become targets for anticancer drugs because of their ligands high specifity and affinity. We focused on the differences between binding of G4-ligands to DNA and their subsequent effect on cells. In the present study, the effects of G4-ligands on tumor (MCF-7) and non-tumor cell lines (HEK293ft) were studied in relation to binding to the DNA and connected mechanisms. Concentration and time-dependent dtudies were performed to study different effects of these compounds. Mentioned cell lines were used to study cell viability after exposure to G4-ligands. Morphological alterations weree observed by fluorescent-based studie

Název v anglickém jazyce

Comparison between synthetic and natural G4-ligands and the effects of their binding to DNA

Popis výsledku anglicky

Among local and non-canonical DNA structures are G-rich sequences that can form G-quadruplex (G4) sequencees widespread qmong prokaryotic and eukaryotic kingdoms and are an increasingly used targets for regulatory proteins. There are evidence from more than three decades of research confirm their function in important cell processes such as transcription and replication. G4s have become targets for anticancer drugs because of their ligands high specifity and affinity. We focused on the differences between binding of G4-ligands to DNA and their subsequent effect on cells. In the present study, the effects of G4-ligands on tumor (MCF-7) and non-tumor cell lines (HEK293ft) were studied in relation to binding to the DNA and connected mechanisms. Concentration and time-dependent dtudies were performed to study different effects of these compounds. Mentioned cell lines were used to study cell viability after exposure to G4-ligands. Morphological alterations weree observed by fluorescent-based studie

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10605 - Developmental biology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů