Increasing PHA production in cyanobacteria by mixotrophic cultivation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F21%3APU142442" target="_blank" >RIV/00216305:26310/21:PU142442 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Increasing PHA production in cyanobacteria by mixotrophic cultivation

Popis výsledku v původním jazyce

Cyanobacteria are phototrophic gram-negative bacteria capable of oxygenic photosynthesis. They are able to synthetize polyhydroxyalkanoates (PHA). They do not require costly organic substrate and, oppositely, cyanobacteria can fix CO2. PHA serve as carbon and energy source in form of intracellular granules. PHA help cyanobacteria to survive stress conditions. In this work we demonstrated that stress conditions lead to the highest yields of PHA. Two cultures from genera Synechocystis were used. Multiple stressors were applied on cultures, stressors include PHA precursors. Cultivation with the same PHA precursors in same concentration led to 1,6-higher amount of PHA when multicultivator was used. The highest amount of PHA reached the 25 % of PHA in dry biomass. Strains are capable of copolymer synthesis in presence of its precursor. Synechocystis sp. PCC 6803 cultivated with γ-butyrolactone accumulated copolymer of 3-hydroxybutyrate (44 %) and 4-hydroxybutyrate (56 %). The total amount of PHA as well as

Název v anglickém jazyce

Increasing PHA production in cyanobacteria by mixotrophic cultivation

Popis výsledku anglicky

Cyanobacteria are phototrophic gram-negative bacteria capable of oxygenic photosynthesis. They are able to synthetize polyhydroxyalkanoates (PHA). They do not require costly organic substrate and, oppositely, cyanobacteria can fix CO2. PHA serve as carbon and energy source in form of intracellular granules. PHA help cyanobacteria to survive stress conditions. In this work we demonstrated that stress conditions lead to the highest yields of PHA. Two cultures from genera Synechocystis were used. Multiple stressors were applied on cultures, stressors include PHA precursors. Cultivation with the same PHA precursors in same concentration led to 1,6-higher amount of PHA when multicultivator was used. The highest amount of PHA reached the 25 % of PHA in dry biomass. Strains are capable of copolymer synthesis in presence of its precursor. Synechocystis sp. PCC 6803 cultivated with γ-butyrolactone accumulated copolymer of 3-hydroxybutyrate (44 %) and 4-hydroxybutyrate (56 %). The total amount of PHA as well as

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GF19-29651L" target="_blank" >GF19-29651L: Ekologická role polyhydroxybutyrátu u cyanobakterií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů